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Açaí (Euterpe oleracea Mart.) presents anti-neuroinflammatory capacity in LPS-activated microglia cells.
de Souza, Diulie Valente; Pappis, Lauren; Bandeira, Thuany Teixeira; Sangoi, Gabriela Geraldo; Fontana, Tuyla; Rissi, Vitor Braga; Sagrillo, Michele Rorato; Duarte, Marta Maria; Duarte, Thiago; Bodenstein, David Frederick; Andreazza, Ana Cristina; Cruz, Ivana Beatrice Mânica da; Ribeiro, Euler Esteves; Antoniazzi, Alfredo; Ourique, Aline Ferreira; Machado, Alencar Kolinski.
Afiliação
  • de Souza DV; Graduate Program in Nanoscience, Franciscan University, Santa Maria, RS, Brazil.
  • Pappis L; Laboratory of Cell Culture and Genetics, Franciscan University, Santa Maria, RS, Brazil.
  • Bandeira TT; Graduate Program of Pharmaceutical Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • Sangoi GG; Laboratory of Cell Culture and Genetics, Franciscan University, Santa Maria, RS, Brazil.
  • Fontana T; Laboratory of Cell Culture and Genetics, Franciscan University, Santa Maria, RS, Brazil.
  • Rissi VB; Laboratory of Cell Culture and Genetics, Franciscan University, Santa Maria, RS, Brazil.
  • Sagrillo MR; Department of Agriculture, Biodiversity and Forests, Federal University of Santa Catarina, Curitibanos, SC, Brazil.
  • Duarte MM; Graduate Program in Nanoscience, Franciscan University, Santa Maria, RS, Brazil.
  • Duarte T; Laboratory of Cell Culture and Genetics, Franciscan University, Santa Maria, RS, Brazil.
  • Bodenstein DF; Graduate Program in Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • Andreazza AC; Graduate Program in Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • Cruz IBMD; Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada.
  • Ribeiro EE; Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada.
  • Antoniazzi A; Centre for Addiction and Mental Health, Toronto, ON, Canada.
  • Ourique AF; Graduate Program in Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil.
  • Machado AK; Third Age Open University, University of Amazonas State, Manaus, AM, Brazil.
Nutr Neurosci ; 25(6): 1188-1199, 2022 Jun.
Article em En | MEDLINE | ID: mdl-33170113
INTRODUCTION: Neuropsychiatric diseases are responsible for one of the highest burden of morbidity and mortality worldwide. These illnesses include schizophrenia, bipolar disorder, and major depression. Individuals affected by these diseases may present mitochondrial dysfunction and oxidative stress. Additionally, patients also have increased peripheral and neural chronic inflammation. The Brazilian fruit, açaí, has been demonstrated to be a neuroprotective agent through its recovery of mitochondrial complex I activity. This extract has previously shown anti-inflammatory effects in inflammatory cells. However, there is a lack of understanding of potential anti-neuroinflammatory mechanisms, such as cell cycle involvement. OBJECTIVE: The objective of this study is to evaluate the anti-neuroinflammatory potential of an açaí extract in lipopolysaccharide-activated BV-2 microglia cells. METHODS: Açaí extract was produced and characterized through high performance liquid chromatography. Following açaí extraction and characterization, BV-2 microglia cells were activated with LPS and a dose-response curve was generated to select the most effective açaí dose to reduce cellular proliferation. This dose was then used to assess reactive oxygen species (ROS) production, double-strand DNA release, cell cycle modulation, and cytokine and caspase protein expression. RESULTS: Characterization of the açaí extract revealed 10 bioactive molecules. The extract reduced cellular proliferation, ROS production, and reduced pro-inflammatory cytokines and caspase 1 protein expression under 1 µg/mL in LPS-activated BV-2 microglia cells but had no effect on double strand DNA release. Additionally, açaí treatment caused cell cycle arrest, specifically within synthesis and G2/Mitosis phases. CONCLUSION: These results suggest that the freeze-dried hydroalcoholic açaí extract presents high anti-neuroinflammatory potential.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Microglia / Euterpe Limite: Animals Idioma: En Revista: Nutr Neurosci Assunto da revista: CIENCIAS DA NUTRICAO / NEUROLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Microglia / Euterpe Limite: Animals Idioma: En Revista: Nutr Neurosci Assunto da revista: CIENCIAS DA NUTRICAO / NEUROLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido