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Changes in Appetite Regulation-Related Signaling Pathways in the Brain of Mice Supplemented with Non-nutritive Sweeteners.
Contreras-Chavez, Gerson G; Estrada, José A; Contreras, Irazú.
Afiliação
  • Contreras-Chavez GG; Laboratorio de Neuroquímica, Facultad de Medicina, Universidad Autónoma del Estado de México, Paseo Tollocan S/N Esq. Jesús Carranza, Colonia Moderna de La Cruz, Estado de México, 50180, Toluca, Mexico.
  • Estrada JA; Laboratorio de Neuroquímica, Facultad de Medicina, Universidad Autónoma del Estado de México, Paseo Tollocan S/N Esq. Jesús Carranza, Colonia Moderna de La Cruz, Estado de México, 50180, Toluca, Mexico.
  • Contreras I; Laboratorio de Neuroquímica, Facultad de Medicina, Universidad Autónoma del Estado de México, Paseo Tollocan S/N Esq. Jesús Carranza, Colonia Moderna de La Cruz, Estado de México, 50180, Toluca, Mexico. icontrerasg@uaemex.mx.
J Mol Neurosci ; 71(6): 1144-1155, 2021 Jun.
Article em En | MEDLINE | ID: mdl-33128194
Non-nutritive sweeteners (NNSs) are commonly used to prevent weight gain and development of metabolic diseases associated with consumption of high-energy diets. Recent studies have demonstrated that these compounds may have unwanted detrimental effects under specific circumstances in vivo. In particular, an association between NNS consumption and changes in signaling pathways involved in the hunger-satiety system in the brain has been reported. Nonetheless, the extent of alterations in brain signaling pathways associated with consumption of these compounds has not been determined. The objective of this study was to determine the effect of frequent consumption of NNSs on the expression of proteins involved in signaling pathways related to appetite control in the brain in vivo. Eight-week-old BALB/c mice were supplemented with sucrose, sucralose, or steviol glycosides in their daily drinking water for 6 weeks. Subsequently, total brain protein extracts were used to analyze the expression of total and phosphorylated JAK2, STAT5, ERK 1/2, JNK, as well as SHP-2 and POMC, by western blot. Serum concentrations of leptin and α-MSH were quantified by ELISA. Results show that consumption of NNSs promotes significant changes in these signaling pathways, reducing the expression of pSTAT5/STAT5, pERK 1/2, SHP-2, and pJNK/JNK in male mice supplemented with steviol glycosides. Furthermore, consumption of steviol glycosides induced a decrease of α-MSH in male mice. In contrast, steviol glycosides induced overexpression of pSTAT5, pERK, and SHP-2 in females. These data suggest that chronic consumption of NNSs promotes sex-specific changes in signaling pathways related to the central hunger-satiety system in vivo.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regulação do Apetite / Encéfalo / Transdução de Sinais / Adoçantes não Calóricos Limite: Animals Idioma: En Revista: J Mol Neurosci Assunto da revista: BIOLOGIA MOLECULAR / NEUROLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regulação do Apetite / Encéfalo / Transdução de Sinais / Adoçantes não Calóricos Limite: Animals Idioma: En Revista: J Mol Neurosci Assunto da revista: BIOLOGIA MOLECULAR / NEUROLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos