Your browser doesn't support javascript.
loading
Study of different routes of immunization using outer membrane vesicles of Neisseria meningitidis B and comparison of two adjuvants.
Brito, Luciana T; Rinaldi, Fabiana M; Gaspar, Emanuelle B; Correa, Victor Araujo; Gonçalves, Caroline A; Portilho, Amanda Izeli; Trzewikoswki de Lima, Gabriela; De Gaspari, Elizabeth.
Afiliação
  • Brito LT; Department of Immunology, Adolfo Lutz Institute, São Paulo, SP, Brazil; Post-Graduate Program Interunities in Biotechnology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
  • Rinaldi FM; Department of Immunology, Adolfo Lutz Institute, São Paulo, SP, Brazil; Post-Graduate Program Interunities in Biotechnology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
  • Gaspar EB; Embrapa Southern Region Animal Husbandry, Bagé, RS, Brazil.
  • Correa VA; Department of Immunology, Adolfo Lutz Institute, São Paulo, SP, Brazil; Post-Graduate Program Interunities in Biotechnology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
  • Gonçalves CA; Department of Immunology, Adolfo Lutz Institute, São Paulo, SP, Brazil.
  • Portilho AI; Department of Immunology, Adolfo Lutz Institute, São Paulo, SP, Brazil; Post-Graduate Program Interunities in Biotechnology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
  • Trzewikoswki de Lima G; Department of Immunology, Adolfo Lutz Institute, São Paulo, SP, Brazil; Post-Graduate Program Interunities in Biotechnology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
  • De Gaspari E; Department of Immunology, Adolfo Lutz Institute, São Paulo, SP, Brazil; Post-Graduate Program Interunities in Biotechnology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil. Electronic address: elizabeth.gaspari@ial.sp.gov.br.
Vaccine ; 38(48): 7674-7682, 2020 11 10.
Article em En | MEDLINE | ID: mdl-33082014
Outer membrane vesicles (OMVs) of Neisseria meningitidis contain important antigens to trigger an immune response against meningococci and have been studied as vaccines compounds. The immune response to a vaccine may be affected by its constitution and route of administration. Therefore, Swiss mice were immunized by different routes with OMVs of N. meningitidis B with dimethyl dioctadecyl ammonium bromide in bilayer fragments (DDA-BF) or aluminum hydroxide (AH) as adjuvants. The adjuvants and different routes were compared regarding the immune responses by ELISA, western blot, delayed type hypersensitivity (DTH) and histopathologic analysis. The antigenic preparation generated humoral and cellular immune responses. In quantitative analyzes, in general, AH was superior to DDA-BF. However, analysis such as IgG avidity index, bactericidal activity and immunoblot, revealed no important differences regarding the adjuvant or route of immunization. Regarding the parameters tested, it was not possible to define a superiority between the adjuvants and routes of immunization proposed by this study.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Anticorpos Antibacterianos / Neisseria meningitidis Limite: Animals Idioma: En Revista: Vaccine Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Anticorpos Antibacterianos / Neisseria meningitidis Limite: Animals Idioma: En Revista: Vaccine Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda