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Polyamidoamine-based nanovector for the efficient delivery of methotrexate to U87 glioma cells.
Ortiz, Natalia; Vásquez, Pilar A; Vidal, Felipe; Díaz, Carola F; Guzmán, José L; Jiménez, Verónica A; Alderete, Joel B.
Afiliação
  • Ortiz N; Departamento de Fisiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción 4030000, Chile.
  • Vásquez PA; Departamento de Fisiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción 4030000, Chile.
  • Vidal F; Departamento de Fisiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción 4030000, Chile.
  • Díaz CF; Departamento de Ciencias Químicas, Facultad de Ciencias Exactas, Universidad Andres Bello, Talcahuano 4260000, Chile.
  • Guzmán JL; Departamento de Fisiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción 4030000, Chile.
  • Jiménez VA; Departamento de Ciencias Químicas, Facultad de Ciencias Exactas, Universidad Andres Bello, Talcahuano 4260000, Chile.
  • Alderete JB; Instituto de Química de Recursos Naturales, Universidad de Talca, Talca 3460000, Chile.
Nanomedicine (Lond) ; 15(28): 2771-2784, 2020 12.
Article em En | MEDLINE | ID: mdl-33073670
The purpose of this study was to design a polyamidoamine (PAMAM)-based nanovector for the efficient delivery of methotrexate to U87 glioma cells. To this end, 0-100% acetylated PAMAM dendrimers of the fourth generation were synthesized and evaluated using drug encapsulation measurements, molecular dynamics simulations, neurotoxicity assays and neuronal internalization experiments. The best system was tested as a nanovector for methotrexate delivery to U87 glioma cells. The authors found that 25% acetylated PAMAM dendrimers of the fourth-generation combine low intrinsic toxicity, large drug complexation capacity and efficient internalization into hippocampal neurons. Nanovector complexation enhances the cytotoxic response of methotrexate against U87 glioma cells compared with free drug solutions. In conclusion, 25% acetylated PAMAM dendrimers of the fourth-generation increase drug uptake by glioma cells and thereby act as efficient nanovectors for methotrexate delivery.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dendrímeros / Glioma Limite: Humans Idioma: En Revista: Nanomedicine (Lond) Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Chile País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dendrímeros / Glioma Limite: Humans Idioma: En Revista: Nanomedicine (Lond) Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Chile País de publicação: Reino Unido