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Long noncoding RNA LINC01189 is associated with HCV-hepatocellular carcinoma and regulates cancer cell proliferation and chemoresistance through hsa-miR-155-5p.
Yao, Ying; Shu, Fang; Wang, Fang; Wang, Xiaoqiang; Guo, Zhengshe; Wang, Haili; Li, Lu; Lv, Haigang.
Afiliação
  • Yao Y; Clinical laboratory, Honghui Hospital, Xi'an Jiaotong University, Xi'an, 710054 Shaanxi, China.
  • Shu F; Clinical laboratory, Third Hospital of Xi'an, 710000, Shaanxi, China.
  • Wang F; Anaesthesiology department, Honghui Hospital, Xi'an Jiaotong University, Xi'an, 710054, Shaanxi, China.
  • Wang X; Department of Cancer Biology, City of Hope National Medical Center, Duarte, CA, 91010, USA.
  • Guo Z; Anaesthesiology department, Honghui Hospital, Xi'an Jiaotong University, Xi'an, 710054, Shaanxi, China.
  • Wang H; Anaesthesiology department, Honghui Hospital, Xi'an Jiaotong University, Xi'an, 710054, Shaanxi, China.
  • Li L; Anaesthesiology department, Honghui Hospital, Xi'an Jiaotong University, Xi'an, 710054, Shaanxi, China.
  • Lv H; Anaesthesiology department, Honghui Hospital, Xi'an Jiaotong University, Xi'an, 710054, Shaanxi, China. Electronic address: doctorlhg@aol.com.
Ann Hepatol ; 22: 100269, 2021.
Article em En | MEDLINE | ID: mdl-33059056
INTRODUCTION AND OBJECTIVES: Emerging evidence has demonstrated that long noncoding RNAs (lncRNAs) may be closely associated with Hepatitis C virus (HCV) infection and the development of hepatocellular carcinoma (HCC). In this study, we investigated the expression and functions of a lncRNA, LINC01189, in HCV-associated HCC. PATIENTS OR MATERIALS AND METHODS: LINC01189 expression was measured in HCC tumors, HCV-infected HCC tumors and HCV-infected HCC cells. LINC01189 was overexpressed in HCV-infected HepG2 cells to measure its function on HCV-correlated cancer proliferation. In HCC cell lines of Huh7 and Hep3B, LINC01189 was upregulated to investigate its effects on cancer cell proliferation and 5-FU chemoresistance. The competing endogenous RNA (ceRNA) target of LINC01189, human microRNA-155-5p (hsa-miR-155-5p) was probed by dual-luciferase assay and qRT-PCR. Hsa-miR-155-5p was upregulated in LINC01189-overexpessed Huh7 and Hep3B cells to investigate their epigenetic correlation on HCC development regulation. RESULTS: LINC01189 is downregulated in HCV-infected HCC tumors and cell lines. LINC01189 overexpression inhibited HCC cancer cell proliferation and 5-FU chemoresistance. Hsa-miR-155-5p was confirmed to be a ceRNA target of LINC01189 in HCC. Upregulating hsa-miR-155-5p reversed the LINC01189-mediated inhibition on HCC proliferation and 5-FU chemoresistance. CONCLUSIONS: LINC01189 downregulation is associated with HCV infection in HCC, and it has tumor-suppressing effects on HCC development through hsa-miR-155-5p.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hepatite C / Carcinoma Hepatocelular / MicroRNAs / RNA Longo não Codificante / Neoplasias Hepáticas Tipo de estudo: Risk_factors_studies Limite: Humans Idioma: En Revista: Ann Hepatol Assunto da revista: GASTROENTEROLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China País de publicação: México
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hepatite C / Carcinoma Hepatocelular / MicroRNAs / RNA Longo não Codificante / Neoplasias Hepáticas Tipo de estudo: Risk_factors_studies Limite: Humans Idioma: En Revista: Ann Hepatol Assunto da revista: GASTROENTEROLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China País de publicação: México