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Virulence and resistance properties of E. coli isolated from urine samples of hospitalized patients in Rio de Janeiro, Brazil - The role of mobile genetic elements.
da Cruz Campos, Ana Carolina; Couto, Natacha; Lucas da Silva Andrade, Nathália; Friedrich, Alex W; de Paula Rosa, Ana Cláudia; Vieira Damasco, Paulo; Chlebowicz-Fliss, Monika A; Rossen, John W A.
Afiliação
  • da Cruz Campos AC; Universidade do Estado do Rio de Janeiro, Faculdade de Ciências Médicas, Departamento de Microbiologia, Imunologia e Parasitologia, Brasil. Blv. 28 de Setembro, 87 fundos 3º andar, 20550-170, Vila Isabel, Rio de Janeiro, Brazil; University of Groningen, University Medical Center Groningen, Departmen
  • Couto N; University of Groningen, University Medical Center Groningen, Department of Medical Microbiology and Infection Prevention, Hanzeplein 1, 9713GZ, Groningen, The Netherlands. Electronic address: n.monge.gomes.do.couto@umcg.nl.
  • Lucas da Silva Andrade N; Universidade do Estado do Rio de Janeiro, Faculdade de Ciências Médicas, Departamento de Microbiologia, Imunologia e Parasitologia, Brasil. Blv. 28 de Setembro, 87 fundos 3º andar, 20550-170, Vila Isabel, Rio de Janeiro, Brazil. Electronic address: nathalu84@yahoo.com.
  • Friedrich AW; University of Groningen, University Medical Center Groningen, Department of Medical Microbiology and Infection Prevention, Hanzeplein 1, 9713GZ, Groningen, The Netherlands. Electronic address: alex.friedrich@umcg.nl.
  • de Paula Rosa AC; Universidade do Estado do Rio de Janeiro, Faculdade de Ciências Médicas, Departamento de Microbiologia, Imunologia e Parasitologia, Brasil. Blv. 28 de Setembro, 87 fundos 3º andar, 20550-170, Vila Isabel, Rio de Janeiro, Brazil. Electronic address: anarosa2004@gmail.com.
  • Vieira Damasco P; Universidade Federal do Estado do Rio de Janeiro, Departamento de Medicina Geral, Rua Mariz e Barros, 775, Tijuca, Rio de Janeiro, 22270-004, Brazil; Universidade do Estado do Rio de Janeiro, Departamento de Doenças Infecciosas e Parasitárias Brasil. Blv 28 de Setembro, 87 fundos 3º andar, 20550-170
  • Chlebowicz-Fliss MA; University of Groningen, University Medical Center Groningen, Department of Medical Microbiology and Infection Prevention, Hanzeplein 1, 9713GZ, Groningen, The Netherlands. Electronic address: m.a.chlebowicz@umcg.nl.
  • Rossen JWA; University of Groningen, University Medical Center Groningen, Department of Medical Microbiology and Infection Prevention, Hanzeplein 1, 9713GZ, Groningen, The Netherlands; ESCMID Study Group for Genomic and Molecular Diagnostics (ESGMD), Basel, Switzerland; University of Utah School of Medicine, De
Int J Med Microbiol ; 310(8): 151453, 2020 Dec.
Article em En | MEDLINE | ID: mdl-33045580
Extraintestinal pathogenic E. coli (ExPEC) is the most frequent etiological agent of urinary tract infections (UTIs). Particular evolutionary successful lineages are associated with severe UTIs and higher incidences of multidrug resistance. Most of the resistance genes are acquired by horizontal transfer of plasmids and other mobile genetic elements (MGEs), and this process has been associated with the successful dissemination of particular lineages. Here, we identified the presence of MGEs and their role in virulence and resistance profiles of isolates obtained from the urine of hospitalized patients in Brazil. Isolates belonging to the successful evolutionary lineages of sequence type (ST) 131, ST405, and ST648 were found to be multidrug-resistant, while those belonging to ST69 and ST73 were often not. Among the ST131, ST405, and ST648 isolates with a resistant phenotype, a high number of mainly IncFII plasmids was identified. The plasmids contained resistance cassettes, and these were also found within phage-related sequences and the chromosome of the isolates. The resistance cassettes were found to harbor several resistance genes, including blaCTX-M-15. In addition, in ST131 isolates, diverse pathogenicity islands similar to those found in highly virulent ST73 isolates were detected. Also, a new genomic island associated with several virulence genes was identified in ST69 and ST131 isolates. In addition, several other MGEs present in the ST131 reference strain EC958 were identified in our isolates, most of them exclusively in ST131 isolates. In contrast, genomic islands present in this reference strain were only partially present or completely absent in our ST131 isolates. Of all isolates studied, ST73 and ST131 isolates had the most similar virulence profile. Overall, no clear association was found between the presence of specific MGEs and virulence profiles. Furthermore, the interplay between virulence and resistance by acquiring MGEs seemed to be lineage dependent. Although the acquisition of IncF plasmids, specific PAIs, GIs, and other MGEs seemed to be involved in the success of some lineages, it cannot explain the success of different lineages, also indicating other (host) factors are involved in this process. Nevertheless, the detection, identification, and surveillance of lineage-specific MGEs may be useful to monitor (new) emerging clones.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Farmacorresistência Bacteriana Múltipla / Infecções por Escherichia coli / Escherichia coli Extraintestinal Patogênica Tipo de estudo: Prognostic_studies Limite: Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Int J Med Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de publicação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Farmacorresistência Bacteriana Múltipla / Infecções por Escherichia coli / Escherichia coli Extraintestinal Patogênica Tipo de estudo: Prognostic_studies Limite: Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Int J Med Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de publicação: Alemanha