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The Potential of IgG to Induce Murine and Human Thymic Maturation of IL-10+ B Cells (B10) Revealed in a Pilot Study.
Inoue, Amanda Harumi Sabô; Lira, Aline Aparecida de Lima; de-Oliveira, Marília Garcia; de Sousa, Thamires Rodrigues; Sgnotto, Fábio da Ressureição; Duarte, Alberto José da Silva; Victor, Jefferson Russo.
Afiliação
  • Inoue AHS; Laboratory of Medical Investigation LIM 56, Division of Dermatology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil.
  • Lira AAL; Laboratory of Medical Investigation LIM 56, Division of Dermatology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil.
  • de-Oliveira MG; Laboratory of Medical Investigation LIM 56, Division of Dermatology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil.
  • de Sousa TR; Laboratory of Medical Investigation LIM 56, Division of Dermatology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil.
  • Sgnotto FDR; Division of Hematology, Medical School, University of Sao Paulo, Sao Paulo 01246-903, Brazil.
  • Duarte AJDS; Laboratory of Medical Investigation LIM 56, Division of Dermatology, Medical School, University of Sao Paulo, Sao Paulo 05403-000, Brazil.
  • Victor JR; Division of Pathology, Medical School, University of Sao Paulo, Sao Paulo 01246-903, Brazil.
Cells ; 9(10)2020 10 05.
Article em En | MEDLINE | ID: mdl-33027887
Regulatory B (B10) cells can control several inflammatory diseases, including allergies; however, the origin of peripheral B10 cells is not fully understood, and the involvement of primary lymphoid organs (PLOs) as a primary site of maturation is not known. Here, using a murine model of allergy inhibition mediated by maternal immunization with ovalbumin (OVA), we aimed to evaluate whether B10 cells can mature in the thymus and whether IgG can mediate this process. Female mice were immunized with OVA, and offspring thymus, bone marrow, spleen, lung, and serum samples were evaluated at different times and after passive transfer of purified IgG or thymocytes. A translational approach was implemented using human nonatopic thymus samples, nonatopic peripheral blood mononuclear cells (PBMCs), and IgG from atopic or nonatopic individuals. Based on the expression of CD1d on B cells during maturation stages, we suggest that B10 cells can also mature in the murine thymus. Murine thymic B10 cells can be induced in vitro and in vivo by IgG and be detected in the spleen and lungs in response to an allergen challenge. Like IgG from atopic individuals, human IgG from nonatopic individuals can induce B10 cells in the infant thymus and adult PBMCs. Our observations suggest that B10 cells may mature in the thymus and that this mechanism may be mediated by IgG in both humans and mice. These observations may support the future development of IgG-based immunoregulatory therapeutic strategies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Imunoglobulina G / Linfócitos B / Interleucina-10 Tipo de estudo: Prognostic_studies Limite: Adult / Animals / Female / Humans Idioma: En Revista: Cells Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Imunoglobulina G / Linfócitos B / Interleucina-10 Tipo de estudo: Prognostic_studies Limite: Adult / Animals / Female / Humans Idioma: En Revista: Cells Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça