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Analysis of cell-biomaterial interaction through cellular bridge formation in the interface between hGMSCs and CaP bioceramics.
Benjumeda Wijnhoven, Isabel; Vallejos, Raúl; Santibanez, Juan F; Millán, Carola; Vivanco, Juan F.
Afiliação
  • Benjumeda Wijnhoven I; Facultad de Artes Liberales, Universidad Adolfo Ibáñez, Viña del Mar, Chile.
  • Vallejos R; Facultad de Ingeniería y Ciencias, Universidad Adolfo Ibáñez, Viña del Mar, Chile.
  • Santibanez JF; Group for Molecular Oncology, Institute for Medical Research, University of Belgrade, Belgrade, Serbia.
  • Millán C; Facultad de Artes Liberales, Universidad Adolfo Ibáñez, Viña del Mar, Chile.
  • Vivanco JF; Facultad de Ingeniería y Ciencias, Universidad Adolfo Ibáñez, Viña del Mar, Chile. juan.vivanco@uai.cl.
Sci Rep ; 10(1): 16493, 2020 10 05.
Article em En | MEDLINE | ID: mdl-33020540
The combination of biomaterials and stem cells for clinical applications constitute a great challenge in bone tissue engineering. Hence, cellular networks derived from cells-biomaterials crosstalk have a profound influence on cell behaviour and communication, preceding proliferation and differentiation. The purpose of this study was to investigate in vitro cellular networks derived from human gingival mesenchymal stem cells (hGMSCs) and calcium phosphate (CaP) bioceramic interaction. Biological performance of CaP bioceramic and hGMSCs interaction was evaluated through cell adhesion and distribution, cellular proliferation, and potential osteogenic differentiation, at three different times: 5 h, 1 week and 4 weeks. Results confirmed that hGMSCs met the required MSCs criteria while displaying osteogenic differentiaton capacities. We found a significant increase of cellular numbers and proliferation levels. Also, protein and mRNA OPN expression were upregulated in cells cultured with CaP bioceramic by day 21, suggesting an osteoinductible effect of the CaP bioceramic on hGMSCs. Remarkably, CaP bioceramic aggregations were obtained through hGMSCs bridges, suggesting the in vitro potential of macrostructures formation. We conclude that hGMSCs and CaP bioceramics with micro and macropores support hGMSC adhesion, proliferation and osteogenic differentiation. Our results suggest that investigations focused on the interface cells-biomaterials are essential for bone tissue regenerative therapies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Materiais Biocompatíveis / Regeneração Óssea / Fosfatos de Cálcio / Comunicação Celular / Células-Tronco Mesenquimais / Gengiva Limite: Humans Idioma: En Revista: Sci Rep Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Chile País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Materiais Biocompatíveis / Regeneração Óssea / Fosfatos de Cálcio / Comunicação Celular / Células-Tronco Mesenquimais / Gengiva Limite: Humans Idioma: En Revista: Sci Rep Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Chile País de publicação: Reino Unido