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B chromosomes of multiple species have intense evolutionary dynamics and accumulated genes related to important biological processes.
Ahmad, Syed F; Jehangir, Maryam; Cardoso, Adauto L; Wolf, Ivan R; Margarido, Vladimir P; Cabral-de-Mello, Diogo C; O'Neill, Rachel; Valente, Guilherme T; Martins, Cesar.
Afiliação
  • Ahmad SF; Department of Structural and Functional Biology, Institute of Bioscience at Botucatu, Sao Paulo State University (UNESP), Botucatu, SP, 18618-689, Brazil.
  • Jehangir M; Department of Structural and Functional Biology, Institute of Bioscience at Botucatu, Sao Paulo State University (UNESP), Botucatu, SP, 18618-689, Brazil.
  • Cardoso AL; Department of Structural and Functional Biology, Institute of Bioscience at Botucatu, Sao Paulo State University (UNESP), Botucatu, SP, 18618-689, Brazil.
  • Wolf IR; Department of Structural and Functional Biology, Institute of Bioscience at Botucatu, Sao Paulo State University (UNESP), Botucatu, SP, 18618-689, Brazil.
  • Margarido VP; Western Paraná State University (UNIOESTE), Center for Biology Science and Health, Cascavel, PR, Brazil.
  • Cabral-de-Mello DC; Department of General and Applied Biology, Institute of Biosciences, Sao Paulo State University (UNESP), Rio Claro, SP, Brazil.
  • O'Neill R; Department of Molecular and Cell Biology, University of Connecticut (UCONN), Storrs, CT, USA.
  • Valente GT; Institute for Systems Genomics, University of Connecticut (UCONN), Storrs, CT, USA.
  • Martins C; Bioprocess and Biotechnology Department, Agronomical Science Faculty, Sao Paulo State University - UNESP, Botucatu, SP, Brazil.
BMC Genomics ; 21(1): 656, 2020 Sep 23.
Article em En | MEDLINE | ID: mdl-32967626
BACKGROUND: One of the biggest challenges in chromosome biology is to understand the occurrence and complex genetics of the extra, non-essential karyotype elements, commonly known as supernumerary or B chromosomes (Bs). The non-Mendelian inheritance and non-pairing abilities of B chromosomes make them an interesting model for genomics studies, thus bringing to bear different questions about their genetic composition, evolutionary survival, maintenance and functional role inside the cell. This study uncovers these phenomena in multiple species that we considered as representative organisms of both vertebrate and invertebrate models for B chromosome analysis. RESULTS: We sequenced the genomes of three animal species including two fishes Astyanax mexicanus and Astyanax correntinus, and a grasshopper Abracris flavolineata, each with and without Bs, and identified their B-localized genes and repeat contents. We detected unique sequences occurring exclusively on Bs and discovered various evolutionary patterns of genomic rearrangements associated to Bs. In situ hybridization and quantitative polymerase chain reactions further validated our genomic approach confirming detection of sequences on Bs. The functional annotation of B sequences showed that the B chromosome comprises regions of gene fragments, novel genes, and intact genes, which encode a diverse set of functions related to important biological processes such as metabolism, morphogenesis, reproduction, transposition, recombination, cell cycle and chromosomes functions which might be important for their evolutionary success. CONCLUSIONS: This study reveals the genomic structure, composition and function of Bs, which provide new insights for theories of B chromosome evolution. The selfish behavior of Bs seems to be favored by gained genes/sequences.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rearranjo Gênico / Cromossomos / Evolução Molecular Limite: Animals Idioma: En Revista: BMC Genomics Assunto da revista: GENETICA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rearranjo Gênico / Cromossomos / Evolução Molecular Limite: Animals Idioma: En Revista: BMC Genomics Assunto da revista: GENETICA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido