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Chronic alcoholism associated with diabetes induced apoptosis in the corpus cavernosum of rats.
Schimming, Bruno Cesar; Cirino, Mucio Luiz de Assis; Lizarte Neto, Fermino Sanches; Novais, Paulo Cezar; Carvalho, Camila Albuquerque Melo de; Tirapelli, Daniela Pretti da Cunha; Molina, Carlos Augusto Fernandes; Tirapelli, Luis Fernando; Tucci Junior, Silvio.
Afiliação
  • Schimming BC; PhD, Associate Professor, Department of Anatomy, Biosciences Institute of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu-SP, Brazil. Technical procedures, interpretation of data, statistical analysis, manuscript preparation, critical revision.
  • Cirino MLA; Fellow PhD degree, Postgraduate Program in Surgical Clinic, Department of Surgery and Anatomy, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo (FMRP-USP), Ribeirao Preto-SP, Brazil. Acquisition and interpretation of data, statistical analysis.
  • Lizarte Neto FS; PhD, Department of Surgery and Anatomy, Surgical Clinic Program, FMRP-USP, Ribeirao Preto-SP, Brazil. Acquisition and interpretation of data, statistical analysis, critical revision.
  • Novais PC; PhD, Department of Odontology, Universidade de Marília (UNIMAR), Marilia-SP, Brazil. Acquisition and interpretation of data, statistical analysis.
  • Carvalho CAM; PhD, Department of Surgery and Anatomy, FMRP-USP, Assistant Professor, Centro Universitário Barão de Mauá, Ribeirao Preto-SP, Brazil. Acquisition and interpretation of data, statistical analysis.
  • Tirapelli DPDC; PhD, Assistant Professor, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, Brazil. Conception and design of the study, critical revision.
  • Molina CAF; PhD, Assistant Professor, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, Brazil. Conception and design of the study, critical revision.
  • Tirapelli LF; PhD, Associate Professor, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, Brazil. Conception and design of the study, critical revision.
  • Tucci Junior S; PhD, Associate Professor, Department of Surgery and Anatomy, FMRP-USP, Ribeirao Preto-SP, Brazil. Conception and design of the study, critical revision, supervised all phases.
Acta Cir Bras ; 35(8): e202000805, 2020 Sep 07.
Article em En | MEDLINE | ID: mdl-32901682
PURPOSE: To evaluate the effects of alcohol exposure and diabetes on apoptotic process in the corpus cavernosum. METHODS: Forty eight male Wistar rats were divided into four groups: control, diabetic, alcoholic and diabetic-alcoholic. Samples of the corpus cavernosum were prepared to study protein expression of apoptotic genes (Caspases-3 and 9) by immunohistochemistry and Real-Time PCR. RESULTS: The immunoreactivity of Caspases-3 and -9 was diffuse and higher in the treated groups though there was no significant difference between the experimental groups, only when compared with the control group. An increase was observed in the gene expression of Caspases-9 in the diabetic and ethanol-diabetic groups when compared with control and ethanol groups. CONCLUSIONS: The association of these factors (ethanol and diabetes) probably can affect the apoptosis mechanism in lesions of the cavernous tissue in the rat penis. Both gene and protein expression of Caspase-9 in diabetic and ethanol-diabetic groups suggest the involvement of the apoptosis cascade from this study model.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Apoptose / Diabetes Mellitus Experimental / Alcoolismo Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: Acta Cir Bras Ano de publicação: 2020 Tipo de documento: Article País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Apoptose / Diabetes Mellitus Experimental / Alcoolismo Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: Acta Cir Bras Ano de publicação: 2020 Tipo de documento: Article País de publicação: Brasil