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Assessment of In Vitro Anti-melanoma Potential of Ephedranthus pisocarpus R.E.Fr.
Santos, Lubna Karine Beserra; Veras, MÁrcia Denise Alves; Marques, Karinne Kelly Gadelha; DE Moraes Alves, Michel MualÉm; Mendes, Anderson Nogueira; DE Amorim Carvalho, Fernando AÉcio; Sobral, Marianna Vieira; Chaves, Mariana Helena; GonÇalves, Juan Carlos Ramos.
Afiliação
  • Santos LKB; Medicinal Plants Research Center, Federal University of Piauí, Teresina, Brazil.
  • Veras MDA; Department of Chemistry, Federal University of Piauí, Teresina, Brazil.
  • Marques KKG; Department of Pharmaceutical Sciences, Federal University of Paraíba, João Pessoa, Brazil.
  • DE Moraes Alves MM; Medicinal Plants Research Center, Federal University of Piauí, Teresina, Brazil.
  • Mendes AN; Department of Veterinary Morphophysiology, Federal University of Piauí, Teresina, Brazil.
  • DE Amorim Carvalho FA; Department of Biophysics and Physiology, Federal University of Piauí, Teresina, Brazil.
  • Sobral MV; Medicinal Plants Research Center, Federal University of Piauí, Teresina, Brazil.
  • Chaves MH; Department of Pharmaceutical Sciences, Federal University of Paraíba, João Pessoa, Brazil.
  • GonÇalves JCR; Department of Chemistry, Federal University of Piauí, Teresina, Brazil.
Anticancer Res ; 40(9): 5015-5024, 2020 Sep.
Article em En | MEDLINE | ID: mdl-32878789
BACKGROUND/AIM: Despite being a rare disease, melanoma is considered the most dangerous skin cancer due to its highly invasive and aggressive nature, and still requires for more effective treatments. The aim of this study was to evaluate the in vitro anti-melanoma potential of Ephedranthus pisocarpus R.E.Fr. (Annonaceae), a popular Brazilian plant with medicinal properties. MATERIALS AND METHODS: Initially, the ethanolic extract (EtOH) was obtained from E. pisocarpus leaves and later partitioned using increasing polarity solvents. The anti-melanoma potential of E. pisocarpus was assessed by spectrophotometry and its cytotoxicity determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and confocal microscopy. RESULTS: We demonstrated that the EtOH extract and fractions from E. pisocarpus had a moderate photoprotective action (FPS 3.0-5.0) against UVA radiation. Interestingly, the dichloromethane fraction presented higher anti-melanoma activity against B16-F10 (IC50=46.8 µg/ml) and SK-MEL-28 cells (IC50=40.1 µg/ml) and lesser toxicity on normal cells. Additionally, our study reported that spathulenol, one of the major constituents from E. pisocarpus, acts through an apoptosis-dependent mechanism in SK-MEL-28 cells. CONCLUSION: The present study demonstrated, for the first time, the in vitro anti-melanoma potential of E. pisocarpus against melanoma cells.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Annonaceae / Antineoplásicos Fitogênicos Limite: Animals / Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Anticancer Res Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Grécia
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Annonaceae / Antineoplásicos Fitogênicos Limite: Animals / Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Anticancer Res Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Grécia