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Diagnostic urinary cfDNA detected in human cystic echinococcosis.
Toribio, Luz; Santivanez, Saul; Scott, Alan L; Enriquez, Raul; Sedano, Cesar; Soto-Becerra, Percy; Garcia, Hector H; Shiff, Clive J.
Afiliação
  • Toribio L; Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru.
  • Santivanez S; Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru; Cysticercosis Unit, Instituto Nacional de Ciencias Neurologicas, Lima, Peru.
  • Scott AL; W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States.
  • Enriquez R; Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru.
  • Sedano C; Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru.
  • Soto-Becerra P; Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru.
  • Garcia HH; Center for Global Health, Universidad Peruana Cayetano Heredia, Lima, Peru; W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States.
  • Shiff CJ; W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States. Electronic address: cshiff1@jhu.edu.
Mol Biochem Parasitol ; 239: 111314, 2020 09.
Article em En | MEDLINE | ID: mdl-32866606
Cystic echinococcosis (CE) is a major neglected tropical zoonotic disease caused by the tissue-dwelling larval stage of the cestode parasite Echinococcus granulosus. For individuals suspected of CE, the diagnostic standard is imaging using ultrasonography, X rays, or computed tomography. These resource-demanding and expensive procedures are rarely available in endemic rural areas where CE is most prevalent. There is a critical need for a new approach to identify CE patients so that they can be managed early in the course of their infection. This study reports on the results of a diagnostic approach that identifies E. granulosus-derived cell-free DNA (cfDNA) in the urine of CE patients. Utilizing PCR to amplify a fragment of a major tandem repeat element found in E. granulosus nuclear DNA, urine samples from all seven imaging-confirmed CE patients who harbored active liver cysts were positive. In addition, the urine samples from 2/4 patients who presented with non-viable/calcified liver cysts were also PCR positive for the repeat fragment. To our knowledge, this is the first report of using parasite cfDNA from urine to diagnose CE. This approach provides an easy to implement and cost-effective method to survey for the prevalence of E. granulosus in humans populations.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Echinococcus granulosus / Equinococose / Ácidos Nucleicos Livres Tipo de estudo: Diagnostic_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Animals / Humans País/Região como assunto: America do sul / Peru Idioma: En Revista: Mol Biochem Parasitol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Peru País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Echinococcus granulosus / Equinococose / Ácidos Nucleicos Livres Tipo de estudo: Diagnostic_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Animals / Humans País/Região como assunto: America do sul / Peru Idioma: En Revista: Mol Biochem Parasitol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Peru País de publicação: Holanda