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Comparison of conventional and extractive fermentation using aqueous two-phase system to extract fibrinolytic proteases produced by Bacillus stearothermophilus DPUA 1729.
Cruz Filho, Raimundo Felipe da; Dos Santos, Januário Gama; Palheta, Rosana Antunes; Santos-Ebinuma, Valéria Carvalho; Viana Marques, Daniela de Araújo; Teixeira, Maria Francisca Simas.
Afiliação
  • Cruz Filho RFD; Culture Collection DPUA/UFAM, Federal University of Amazonas, Manaus, Brazil.
  • Dos Santos JG; Culture Collection DPUA/UFAM, Federal University of Amazonas, Manaus, Brazil.
  • Palheta RA; Culture Collection DPUA/UFAM, Federal University of Amazonas, Manaus, Brazil.
  • Santos-Ebinuma VC; Department of Bioprocess and Biotechnology, School of Pharmaceutical Sciences, UNESP-University Estadual Paulista, Araraquara, Brazil.
  • Viana Marques DA; Laboratory of Biotechnology Applied to Infectious and Parasitic Diseases, Biological Science Institute, University of Pernambuco, Santo Amaro, Brazil.
  • Teixeira MFS; Culture Collection DPUA/UFAM, Federal University of Amazonas, Manaus, Brazil.
Prep Biochem Biotechnol ; 51(2): 191-200, 2021.
Article em En | MEDLINE | ID: mdl-32845203
Fibrinolytic enzymes have been considered promising for treatment and protection of healthy circulation due its ability to dissolve the fibrin in blood clots. Extractive fermentation is a not explored and efficient downstream process which segregates the desired product simultaneously in a fermentation process fast and economically. Extraction of fibrinolytic enzymes by Bacillus stearothermophilus DPUA 1729 employing conventional aqueous two-phase systems (ATPS) and extractive fermentation with ATPS was evaluated. The results of both systems were compared using a factorial design with PEG molar mass, PEG and salt concentrations as independent variables and extraction parameters as a response. In all conditions evaluated it was observed a similar partitioning of fibrinolytic enzymes through the phases, both in conventional ATPS and extractive fermentation. Salt concentration and interaction among PEG and salt concentration influenced in the partition coefficient. The fibrinolytic activity was determined by hydrolysis of fibrin in plate using the extract of one condition from extractive fermentation. The zone degradation presented a diameter of 7.03 ± 0.94 mm. In conclusion, there was no significant difference among the results obtained using conventional ATPS and extractive fermentation, however, the second one presents more advantages and can integrate production and extraction in one single step, reducing the costs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeo Hidrolases / Trombose / Geobacillus stearothermophilus / Fermentação Limite: Animals Idioma: En Revista: Prep Biochem Biotechnol Assunto da revista: BIOQUIMICA / BIOTECNOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peptídeo Hidrolases / Trombose / Geobacillus stearothermophilus / Fermentação Limite: Animals Idioma: En Revista: Prep Biochem Biotechnol Assunto da revista: BIOQUIMICA / BIOTECNOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido