Your browser doesn't support javascript.
loading
Gyroxin, a toxin from Crotalus durissus terrificus snake venom, induces a calcium dependent increase in glutamate release in mice brain cortical synaptosomes.
Ferrari, C Z; Ribeiro, R; Lima, A M; Soares, A M; Cavalcante, W L G; Vieira, L B.
Afiliação
  • Ferrari CZ; Department of Pharmacology, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Ribeiro R; Department of Pharmacology, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Lima AM; Laboratório de Biotecnologia de Proteínas e Compostos Bioativos da Amazônia Ocidental, LaBioProt, Centro de Estudos de Biomoléculas Aplicadas à Saúde, CEBio, Fundação Oswaldo Cruz, FIOCRUZ, Fiocruz Rondônia e Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil.
  • Soares AM; Laboratório de Biotecnologia de Proteínas e Compostos Bioativos da Amazônia Ocidental, LaBioProt, Centro de Estudos de Biomoléculas Aplicadas à Saúde, CEBio, Fundação Oswaldo Cruz, FIOCRUZ, Fiocruz Rondônia e Universidade Federal de Rondônia, UNIR, Porto Velho, RO, Brazil; Centro Universitário São L
  • Cavalcante WLG; Department of Pharmacology, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. Electronic address: wcavacalnte@icb.ufmg.br.
  • Vieira LB; Department of Pharmacology, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. Electronic address: lubvieira@icb.ufmg.br.
Neuropeptides ; 83: 102081, 2020 Oct.
Article em En | MEDLINE | ID: mdl-32839009
Gyroxin is a thrombin-like toxin obtained from the venom of the South American rattlesnake, Crotalus durissus terrificus. Literature has reported "gyroxin syndrome" characterized, in mice, as series of aberrant motor behavior, known as barrel rotation, mainly after intraperitoneal administration. Despites several studies, a physiological mechanism of "gyroxin syndrome" are still not completely understood. In this context, alterations on the central nervous system (CNS), especially causing neurotoxic events, are pointed out as likely candidates. Then, we decided to investigate whether gyroxin induces alterations in glutamate release, one of the most important neurotransmitter involved in neurotoxicity. For that, we performed all experiments, in vitro, using a model of mice brain cortical synaptosomes. Notably, our results indicate that the administration of gyroxin on purified presynaptic brain cortical terminals resulted in an extracellular Ca2+- dependent raise in glutamate release. Indeed, our results also showed that gyroxin increases intrasynaptosomal calcium (Ca2+) levels through acting on voltage gated calcium channels (VGCC), specifically N and P/Q subtypes. Moreover, our data show that gyroxin increases exocytosis rate. Interestingly, these data suggest that gyroxin might induce neurotoxicity by increasing glutamate levels. However, future investigations are needed in order to elucidate the nature of the following events.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sinaptossomos / Córtex Cerebral / Cálcio / Ácido Glutâmico / Venenos de Crotalídeos / Neurotoxinas Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Neuropeptides Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sinaptossomos / Córtex Cerebral / Cálcio / Ácido Glutâmico / Venenos de Crotalídeos / Neurotoxinas Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Neuropeptides Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda