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Incidence and characteristics of methicillin-resistant coagulase-negative Staphylococcus aureus in peritoneal dialysis-associated peritonitis in a single center using molecular methods.
Camargo, Carlos Henrique; de Souza da Cunha, Maria de Lourdes Ribeiro; Caramori, Jacqueline Costa Teixeira; Mondelli, Alessandro Lia; Montelli, Augusto Cezar; Barretti, Pasqual.
Afiliação
  • Camargo CH; Department of Internal Medicine, Botucatu Medical School, Sao Paulo State University, Botucatu, São Paulo, Brazil. carlos.camargo@ial.sp.gov.br.
  • de Souza da Cunha MLR; Department of Microbiology and Immunology, Biosciences Institute of Botucatu, Sao Paulo State University, Botucatu, São Paulo, Brazil. carlos.camargo@ial.sp.gov.br.
  • Caramori JCT; Bacteriology Division, Instituto Adolfo Lutz, Avenida Dr. Arnaldo, 351 - 9° Andar, Pacaembú, São Paulo, SP, Brasil. carlos.camargo@ial.sp.gov.br.
  • Mondelli AL; Department of Microbiology and Immunology, Biosciences Institute of Botucatu, Sao Paulo State University, Botucatu, São Paulo, Brazil.
  • Montelli AC; Department of Internal Medicine, Botucatu Medical School, Sao Paulo State University, Botucatu, São Paulo, Brazil.
  • Barretti P; Department of Internal Medicine, Botucatu Medical School, Sao Paulo State University, Botucatu, São Paulo, Brazil.
Int Urol Nephrol ; 53(2): 373-380, 2021 Feb.
Article em En | MEDLINE | ID: mdl-32804344
PURPOSE: Peritonitis is a serious complication of peritoneal dialysis and coagulase-negative Staphylococcus (CNS) is the most frequent cause of peritoneal dialysis (PD)-infections in many centers. This study aimed to investigate the molecular epidemiology of CNS isolated from PD-peritonitis in a Brazilian single center, focusing on the genetic determinants conferring methicillin resistance. METHODS: Bacterial strains were isolated from peritoneal fluid of patients presenting PD-peritonitis, identified by phenotypic and molecular methods, and those identified as CNS were submitted to mecA detection, SCCmec, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). RESULTS: Over the 18-year period of this study (1995-2011), a total of 878 peritonitis episodes were diagnosed in this unit, 115 were caused by coagulase-negative staphylococci of which 72 by Staphylococcus epidermidis. mecA gene was detected in 55 CNS (47.8%), more frequently on the more recent years. SCCmec type III was the most frequent cassette, followed by SCCmec type IV and SCCmec type II. A diverstity of pulsotypes was observed among the S. epidermidis isolates, but five clusters (based on the 80% cutoff) were identified. Diversified sequence types (ST02, ST05, ST06, ST09, ST23, ST59 and ST371) were detected. CONCLUSIONS: Detection of SCCmec type III among coagulase-negative Staphylococcus underscores the role of hospital environments as potential source of methicillin-resistant Staphylococcus causing peritonitis in PD patients.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peritonite / Infecções Estafilocócicas / Staphylococcus epidermidis / Diálise Peritoneal / Staphylococcus aureus Resistente à Meticilina Tipo de estudo: Diagnostic_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: Int Urol Nephrol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peritonite / Infecções Estafilocócicas / Staphylococcus epidermidis / Diálise Peritoneal / Staphylococcus aureus Resistente à Meticilina Tipo de estudo: Diagnostic_studies / Incidence_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Humans Idioma: En Revista: Int Urol Nephrol Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda