siRNA Design to Silence the 3'UTR Region of Zika Virus.
Biomed Res Int
; 2020: 6759346, 2020.
Article
em En
| MEDLINE
| ID: mdl-32802864
The disease caused by the Zika virus (ZIKV) has positioned itself as one of the main public health problems in Mexico. One of the main reasons is it causes microcephaly and other birth defects. The transmission of ZIKV is through Aedes aegypti and Ae. albopictus mosquitoes, which are found in a larger space of the national territory. In addition, it can also be transmitted via blood transfusion, sexual relations, and maternal-fetal route. So far, there are no vaccines or specific treatments to deal with this infection. Currently, some new therapeutics such as small interfering RNAs (siRNAs) are able to regulate or suppress transcription in viruses. Therefore, in this project, an in silico siRNA was designed for the 3'UTR region of ZIKV via bioinformatics tools. The designed siRNA was synthesized and transfected into the C6/36 cell line, previously infected with ZIKV in order to assess the ability of the siRNA to inhibit viral replication. The designed siRNA was able to inhibit significantly (p < 0.05) ZIKV replication; this siRNA could be considered a potential therapeutic towards the disease that causes ZIKV and the medical problems generated.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Replicação Viral
/
RNA Viral
/
Regiões 3' não Traduzidas
/
RNA Interferente Pequeno
/
Zika virus
Limite:
Humans
Idioma:
En
Revista:
Biomed Res Int
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
México
País de publicação:
Estados Unidos