Biotransformation in the fish Prochilodus lineatus: An organ-specific approach to cyp1a gene expression and biochemical activity.
Environ Toxicol Pharmacol
; 80: 103467, 2020 Nov.
Article
em En
| MEDLINE
| ID: mdl-32791344
The biotransformation ability of the organism is the result of organ-specific responses. This paper presents a molecular and biochemical approach to elucidate the biotransformation mechanisms in different organs of Prochilodus lineatus induced at 6, 24, and 96â¯h after a benzo[a]pyrene (B[a]P) injection. The induction in cyp1a transcription showed an organ-specific intensity at every tested time time. The EROD (ethoxyresorufin-O-deethylase) activity increased rapidly (6â¯h) in the liver and the kidney; the gills and the brain showed an increase at 24â¯h; and the gills demonstrated the highest activity among all the organs tested. There was no increase in glutathione S-transferase (GST) activity or lipoperoxidation. The decreased hepatic glutathione content (GSH) may be due to its role as an antioxidant. B[a]P was detected in the bile, confirming the xenobiotic efflux from the metabolizing organs. The gills, liver, brain, and kidney of P. lineatus presented an integrated mechanism to deal with the xenobiotic biotransformation.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Benzo(a)pireno
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Citocromo P-450 CYP1A1
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Caraciformes
Limite:
Animals
Idioma:
En
Revista:
Environ Toxicol Pharmacol
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Holanda