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In vivo Regeneration of Mineralized Bone Tissue in Anisotropic Biomimetic Sponges.
Serrano-Bello, Janeth; Cruz-Maya, Iriczalli; Suaste-Olmos, Fernando; González-Alva, Patricia; Altobelli, Rosaria; Ambrosio, Luigi; Medina, Luis Alberto; Guarino, Vincenzo; Alvarez-Perez, Marco Antonio.
Afiliação
  • Serrano-Bello J; Tissue Bioengineering Laboratory, Postgraduate Studies and Research Division, Faculty of Dentistry, National Autonomous University of Mexico, Mexico City, Mexico.
  • Cruz-Maya I; Tissue Bioengineering Laboratory, Postgraduate Studies and Research Division, Faculty of Dentistry, National Autonomous University of Mexico, Mexico City, Mexico.
  • Suaste-Olmos F; Institute of Polymers, Composites, and Biomaterials, National Research Council of Italy, Naples, Italy.
  • González-Alva P; Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, Mexico.
  • Altobelli R; Tissue Bioengineering Laboratory, Postgraduate Studies and Research Division, Faculty of Dentistry, National Autonomous University of Mexico, Mexico City, Mexico.
  • Ambrosio L; Institute of Composite and Biomedical Materials, National Research Council of Italy, Naples, Italy.
  • Medina LA; Institute of Polymers, Composites, and Biomaterials, National Research Council of Italy, Naples, Italy.
  • Guarino V; Instituto de Física, Universidad Nacional Autónoma de México, Mexico City, Mexico.
  • Alvarez-Perez MA; Unidad de Investigación Biomédica en Cáncer, Instituto Nacional de Cancerología/Universidad Nacional Autónoma de México, Mexico City, Mexico.
Article em En | MEDLINE | ID: mdl-32775319
In the last two decades, alginate scaffolds have been variously studied as extracellular matrix analogs for tissue engineering. However, relevant evidence is still lacking concerning their ability to mimic the microenvironment of hierarchical tissues such as bone. Hence, an increasing amount of attention has recently been devoted to the fabrication of macro/microporous sponges with pore anisotropy able to more accurately replicate the cell niche structure as a trigger for bioactive functionalities. This paper presents an in vivo study of alginate sponges with anisotropic microporous domains (MAS) formed by ionic crosslinking in the presence of different fractions (30 or 50% v) of hydroxyapatite (HA). In comparison with unloaded sponges (MAS0), we demonstrated that HA confers peculiar physical and biological properties to the sponge, depending upon the inorganic fraction used, enabling the sponge to bio-mimetically support the regeneration of newly formed bone. Scanning electron microscopy analysis showed a preferential orientation of pores, ascribable to the physical constraints exerted by HA particles during the pore network formation. Energy dispersive spectroscopy (EDS) and X-Ray diffraction (XRD) confirmed a chemical affinity of HA with the native mineral phase of the bone. In vitro studies via WST-1 assay showed good adhesion and proliferation of human Dental Pulp-Mesenchymal Stem Cells (hDP-MSC) that increased in the presence of the bioactive HA signals. Moreover, in vivo studies via micro-CT and histological analyses of a bone model (e.g., a rat calvaria defect) confirmed that the maximum osteogenic response after 90 days was achieved with MAS30, which supported good regeneration of the calvaria defect without any evidence of inflammatory reaction. Hence, all of the results suggested that MAS is a promising scaffold for supporting the regeneration of hard tissues in different body compartments.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Front Bioeng Biotechnol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: México País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Front Bioeng Biotechnol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: México País de publicação: Suíça