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Molecular evidence of Anaplasma phagocytophilum and Theileria equi coinfection in horses from Rio de Janeiro, Brazil.
Dos Santos, Tiago Marques; Roier, Erica Cristina Rocha; Pires, Marcus Sandes; Santos, Huarrisson Azevedo; Vilela, Joice Aparecida Rezende; Peckle, Maristela; Paulino, Patrícia Gonzaga; Baldani, Cristiane Divan; Massard, Carlos Luiz.
Afiliação
  • Dos Santos TM; Department of Epidemiology and Public Health, Veterinary Institute, Federal Rural University of Rio de Janeiro, Br 465, km 7, Seropedica, Rio de Janeiro 23897-000, Brazil.
  • Roier ECR; Department of Veterinary Medicine, Severino Sombra University, Vassouras, Rio de Janeiro, Brazil.
  • Pires MS; Department of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, Br 465, km 7, Seropedica, Rio de Janeiro 23897-000, Brazil.
  • Santos HA; Department of Epidemiology and Public Health, Veterinary Institute, Federal Rural University of Rio de Janeiro, Br 465, km 7, Seropedica, Rio de Janeiro 23897-000, Brazil.
  • Vilela JAR; Company of Technical Assistance and Extension Rural (EMATER) Estrada RJ 99, km 8 - Piranema, Itaguaí, Rio de Janeiro 23855-120, Brazil.
  • Peckle M; Department of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, Br 465, km 7, Seropedica, Rio de Janeiro 23897-000, Brazil.
  • Paulino PG; Department of Epidemiology and Public Health, Veterinary Institute, Federal Rural University of Rio de Janeiro, Br 465, km 7, Seropedica, Rio de Janeiro 23897-000, Brazil.
  • Baldani CD; Department of Medicine and Veterinary Surgery, Federal Rural University of Rio de Janeiro, BR 465, Km 7, Seropédica, Rio de Janeiro 23890000, Brazil.
  • Massard CL; Department of Animal Parasitology, Veterinary Institute, Federal Rural University of Rio de Janeiro, Br 465, km 7, Seropedica, Rio de Janeiro 23897-000, Brazil.
Vet Anim Sci ; 7: 100055, 2019 Jun.
Article em En | MEDLINE | ID: mdl-32734076
The present study aims to determine the frequencies of Theileria equi and Anaplasma phagocytophilum antibodies among horses from the state of Rio de Janeiro, Brazil, and to detect the presence of DNA of these pathogens through molecular methods. A total of 98 serum samples of horses from the municipality of Seropedica were tested by indirect immunofluorescence antibody (IFA) to detect anti-A. phagocytophilum and anti-T. equi IgG antibodies. In addition, quantitative real-time PCR (qPCR) was used to detect these pathogens in the DNA extracted from the whole blood and buffy coat of horses. Bivariate analysis and odds ratio were performed to verify the possible association between positivity and characteristics related to the horses. As evaluated by IFA and qPCR, the frequency of animals that tested positive for T. equi was 89.8% (n = 88/98) and 91.8% (n = 90/98), whereas A. phagocytophilum was 17.4% (n = 17/98) and 1.0% (n = 1/98), respectively. Serological evidence of exposure to A. phagocytophilum and T. equi was observed in 16.3% (n = 16/98) of the horses; however, exposure was confirmed by qPCR in only 1.0% (n = 1/98). No statistical association was found in the bivariate and odds ratio analysis. This is the first study reporting the molecular detection of A. phagocytophilum DNA in horses from the state of Rio de Janeiro, and also the coinfection of A. phagocytophilum and T. equi in a horse from Brazil confirmed by molecular methods. Equine granulocytic anaplasmosis is circulating in Brazilian horses, together with T. equi, and should be included in the differential diagnosis of tick-borne diseases.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE País/Região como assunto: America do sul / Brasil Idioma: En Revista: Vet Anim Sci Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE País/Região como assunto: America do sul / Brasil Idioma: En Revista: Vet Anim Sci Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda