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Novel fibrin-fibronectin matrix accelerates mice skin wound healing.
Jara, Carlos Poblete; Wang, Ou; Paulino do Prado, Thais; Ismail, Ayman; Fabian, Frank Marco; Li, Han; Velloso, Licio A; Carlson, Mark A; Burgess, William; Lei, Yuguo; Velander, William H; Araújo, Eliana P.
Afiliação
  • Jara CP; Nursing School, University of Campinas, Campinas, SP, Brazil.
  • Wang O; Laboratory of Cell Signaling, University of Campinas, Campinas, SP, Brazil.
  • Paulino do Prado T; Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, NE, USA.
  • Ismail A; Biomedical Engineering Program, University of Nebraska, Lincoln, NE, USA.
  • Fabian FM; Nursing School, University of Campinas, Campinas, SP, Brazil.
  • Li H; Laboratory of Cell Signaling, University of Campinas, Campinas, SP, Brazil.
  • Velloso LA; Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, NE, USA.
  • Carlson MA; Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, NE, USA.
  • Burgess W; Department of Chemical and Biomolecular Engineering, University of Nebraska, Lincoln, NE, USA.
  • Lei Y; School of Biological Science, University of Nebraska, Lincoln, NE, USA.
  • Velander WH; Laboratory of Cell Signaling, University of Campinas, Campinas, SP, Brazil.
  • Araújo EP; Department of Surgery, University of Nebraska Medical Center and the VA Nebraska-Western Iowa Health Care System, USA.
Bioact Mater ; 5(4): 949-962, 2020 Dec.
Article em En | MEDLINE | ID: mdl-32671290
Plasma fibrinogen (F1) and fibronectin (pFN) polymerize to form a fibrin clot that is both a hemostatic and provisional matrix for wound healing. About 90% of plasma F1 has a homodimeric pair of γ chains (γγF1), and 10% has a heterodimeric pair of γ and more acidic γ' chains (γγ'F1). We have synthesized a novel fibrin matrix exclusively from a 1:1 (molar ratio) complex of γγ'F1 and pFN in the presence of highly active thrombin and recombinant Factor XIII (rFXIIIa). In this matrix, the fibrin nanofibers were decorated with pFN nanoclusters (termed γγ'F1:pFN fibrin). In contrast, fibrin made from 1:1 mixture of γγF1 and pFN formed a sporadic distribution of "pFN droplets" (termed γγF1+pFN fibrin). The γγ'F1:pFN fibrin enhanced the adhesion of primary human umbilical vein endothelium cells (HUVECs) relative to the γγF1+FN fibrin. Three dimensional (3D) culturing showed that the γγ'F1:pFN complex fibrin matrix enhanced the proliferation of both HUVECs and primary human fibroblasts. HUVECs in the 3D γγ'F1:pFN fibrin exhibited a starkly enhanced vascular morphogenesis while an apoptotic growth profile was observed in the γγF1+pFN fibrin. Relative to γγF1+pFN fibrin, mouse dermal wounds that were sealed by γγ'F1:pFN fibrin exhibited accelerated and enhanced healing. This study suggests that a 3D pFN presentation on a fibrin matrix promotes wound healing.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Bioact Mater Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: China
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Bioact Mater Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: China