Your browser doesn't support javascript.
loading
Breaks of macrosynteny and collinearity among moth bean (Vigna aconitifolia), cowpea (V. unguiculata), and common bean (Phaseolus vulgaris).
Oliveira, Ana Rafaela da S; Martins, Lívia do Vale; Bustamante, Fernanda de O; Muñoz-Amatriaín, María; Close, Timothy; da Costa, Antônio F; Benko-Iseppon, Ana Maria; Pedrosa-Harand, Andrea; Brasileiro-Vidal, Ana Christina.
Afiliação
  • Oliveira ARDS; Departamento de Genética, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • Martins LDV; Departamento de Genética, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • Bustamante FO; Departamento de Genética, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • Muñoz-Amatriaín M; Department of Soil and Crop Sciences, Colorado State University, Fort Collins, CO, USA.
  • Close T; Department of Botany and Plant Sciences, University of California, Riverside, CA, USA.
  • da Costa AF; Instituto Agronômico de Pernambuco, Recife, Pernambuco, Brazil.
  • Benko-Iseppon AM; Departamento de Genética, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • Pedrosa-Harand A; Departamento de Botânica, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil.
  • Brasileiro-Vidal AC; Departamento de Genética, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil. brasileirovidal.ac@gmail.com.
Chromosome Res ; 28(3-4): 293-306, 2020 12.
Article em En | MEDLINE | ID: mdl-32654079
Comparative cytogenetic mapping is a powerful approach to gain insights into genome organization of orphan crops, lacking a whole sequenced genome. To investigate the cytogenomic evolution of important Vigna and Phaseolus beans, we built a BAC-FISH (fluorescent in situ hybridization of bacterial artificial chromosome) map of Vigna aconitifolia (Vac, subgenus Ceratotropis), species with no sequenced genome, and compared with V. unguiculata (Vu, subgenus Vigna) and Phaseolus vulgaris (Pv) maps. Seventeen Pv BACs, eight Vu BACs, and 5S and 35S rDNA probes were hybridized in situ on the 11 Vac chromosome pairs. Five Vac chromosomes (Vac6, Vac7, Vac9, Vac10, and Vac11) showed conserved macrosynteny and collinearity between V. unguiculata and P. vulgaris. On the other hand, we observed collinearity breaks, identified by pericentric inversions involving Vac2 (Vu2), Vac4 (Vu4), and Vac3 (Pv3). We also detected macrosynteny breaks of translocation type involving chromosomes 1 and 8 of V. aconitifolia and P. vulgaris; 2 and 3 of V. aconitifolia and P. vulgaris; and 1 and 5 of V. aconitifolia and V. unguiculata. Considering our data and previous BAC-FISH studies, six chromosomes (1, 2, 3, 4, 5, and 8) are involved in major karyotype divergences between genera and five (1, 2, 3, 4, and 5) between Vigna subgenera, including mechanisms such as duplications, inversions, and translocations. Macrosynteny breaks between Vigna and Phaseolus suggest that the major chromosomal rearrangements have occurred within the Vigna clade. Our cytogenomic comparisons bring new light on the degree of shared macrosynteny and mechanisms of karyotype diversification during Vigna and Phaseolus evolution.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Genômica / Phaseolus / Citogenética / Vigna Idioma: En Revista: Chromosome Res Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Genômica / Phaseolus / Citogenética / Vigna Idioma: En Revista: Chromosome Res Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda