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Prevention of Prostate Cancer in Transgenic Adenocarcinoma of the Mouse Prostate Mice by Yellow Passion Fruit Extract and Antiproliferative Effects of Its Bioactive Compound Piceatannol.
Kido, Larissa Akemi; Hahm, Eun-Ryeong; Kim, Su-Hyeong; Baseggio, Andressa Mara; Cagnon, Valeria Helena Alves; Singh, Shivendra V; Maróstica, Mário Roberto.
Afiliação
  • Kido LA; Department of Food and Nutrition, Faculty of Food Engineering, University of Campinas, Campinas, Brazil.
  • Hahm ER; Department of Structural and Functional Biology, Institute of Biology, University of Campinas, Campinas, Brazil.
  • Kim SH; Department of Pharmacology and Chemical Biology, PA, USA.
  • Baseggio AM; Department of Pharmacology and Chemical Biology, PA, USA.
  • Cagnon VHA; Department of Food and Nutrition, Faculty of Food Engineering, University of Campinas, Campinas, Brazil.
  • Singh SV; Department of Structural and Functional Biology, Institute of Biology, University of Campinas, Campinas, Brazil.
  • Maróstica MR; Department of Pharmacology and Chemical Biology, PA, USA.
J Cancer Prev ; 25(2): 87-99, 2020 Jun 30.
Article em En | MEDLINE | ID: mdl-32647650
Piceatannol (PIC), a polyphenol presents in many vegetables and fruits including yellow passion fruit extract (PFE; Passiflora edulis), has anti-cancer activity, but its molecular targets are still poorly understood. The aims of this study were to investigate the molecular mechanistic actions of PIC in prostate cancer cell lines and to test if the extract from PFE rich in PIC can affect the growth of prostate cancer cells in the Transgenic Adenocarcinoma of the Mouse Prostate (TRAMP) model. The PC-3, 22Rv1, LNCaP, and VCaP prostate cancer cells were exposed to PIC (10-40 µM), and cell viability, lactate measurement, Western blot, and flow cytometric analyses were performed. For an in vivo experiments, eight-week-old TRAMP mice (n = 10 per group each) received an aqueous extract of PFE containing 20 mg of PIC/kg or water (control group) by gavage for 4 or 10 weeks for further analyses. PIC treatment concentration- and time-dependently reduced viability of all cell lines tested. 22Rv1 and LNCaP cells treated with PIC did not exhibit any significant alteration in the intracellular accumulation of lactate. PIC treatment caused G0/G1 phase cell cycle arrest and induction of apoptosis in both LNCaP and 22Rv1 cells. PIC-treated cells exhibited altered protein levels of p53, p21, cyclin D1, and cyclin-dependent kinase 4 (cdk4). The short and long-term PFE treatments also affected p21, cyclin D1 and cdk4 and delayed disease progression in TRAMP, with a decreased incidence of preneoplastic lesions. In conclusion, PIC apparently does not alter glucose metabolism in prostate cancer cells, while cell cycle arrest and p53 modulation are likely important in anti-cancer effects of PIC alone or as a food matrix byproduct in prostate cancer cells, especially those with an androgen-dependent phenotype.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: J Cancer Prev Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação:
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: J Cancer Prev Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: