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Cytotoxic Effect of Niobium Phosphate Glass-based Gutta-Percha Points on Periodontal Ligament Fibroblasts In Vitro.
Meneses, Claudia Caroline Bosio; Olivi, Lucas Tofanello; Carvalho, Ceci Nunes; Gavini, Giulio; Sipert, Carla Renata.
Afiliação
  • Meneses CCB; Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, São Paulo, Brazil.
  • Olivi LT; Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, São Paulo, Brazil.
  • Carvalho CN; School of Dentistry, University Universidade do Centro de Estudos Unificados do Maranhão - CEUMA, São Luiz, Maranhão, Brazil.
  • Gavini G; Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, São Paulo, Brazil.
  • Sipert CR; Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, São Paulo, Brazil. Electronic address: sipert@usp.br.
J Endod ; 46(9): 1297-1301, 2020 Sep.
Article em En | MEDLINE | ID: mdl-32615173
INTRODUCTION: The outcome of root canal obturation might be affected by the chemical components of the chosen filling materials. Niobium phosphate glass-based gutta-percha (GNB) was proposed as a biomaterial-based obturation point. This study aimed to investigate the cytotoxic and cell modulation effects of GNB points on human periodontal ligament fibroblasts (PDLFs) in vitro. METHODS: Human PDLFs were cultured for the assays. Extracts of regular gutta-percha (GP) points and GNB were obtained, serially diluted (1:5, 1:10, and 1:25), and used to stimulate PDLFs. A cell viability assay was performed using alamarBlue reagent (Molecular Probes, Waltham, MA), and reverse transcription quantitative polymerase chain reaction was used to assess the gene expression for collagen type I and cementum protein 1. One-way analysis of variance followed by the Tukey post hoc test was performed (P < .05). RESULTS: Regular GP reduced cell viability only in pure extracts, whereas GNB exhibited cytotoxicity to PDLFs in pure extracts as well as 1/5 and 1/10 dilutions. The gene expression of collagen type I was down-regulated only in the GNB group (P < .05). The expression of cementum protein 1 remained unaltered by both tested materials. CONCLUSIONS: The addition of niobium phosphate glass to GP points increased cytotoxicity, affecting PDLF viability and partially disturbing physiological cell function.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Materiais Restauradores do Canal Radicular / Guta-Percha Limite: Humans Idioma: En Revista: J Endod Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Materiais Restauradores do Canal Radicular / Guta-Percha Limite: Humans Idioma: En Revista: J Endod Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos