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Development of chitosan nanocapsules containing essential oil of Matricaria chamomilla L. for the treatment of cutaneous leishmaniasis.
Karam, Thaysa Ksiaskiewcz; Ortega, Sonia; Ueda Nakamura, Tania; Auzély-Velty, Rachel; Nakamura, Celso Vataru.
Afiliação
  • Karam TK; State University of Maringá, Laboratory of Microbiology Applied to Natural and Synthetic Products, Department of Pharmaceutical Sciences, Maringá, Brazil.
  • Ortega S; University Grenoble Alpes, Centre de Recherche de Macromolecules Végetales, CERMAV - CNRS, Grenoble, France.
  • Ueda Nakamura T; State University of Maringá, Laboratory of Microbiology Applied to Natural and Synthetic Products, Department of Pharmaceutical Sciences, Maringá, Brazil.
  • Auzély-Velty R; University Grenoble Alpes, Centre de Recherche de Macromolecules Végetales, CERMAV - CNRS, Grenoble, France. Electronic address: rachel.auzely@cermav.cnrs.fr.
  • Nakamura CV; State University of Maringá, Laboratory of Microbiology Applied to Natural and Synthetic Products, Department of Pharmaceutical Sciences, Maringá, Brazil. Electronic address: cvnakamura@gmail.com.
Int J Biol Macromol ; 162: 199-208, 2020 Nov 01.
Article em En | MEDLINE | ID: mdl-32565304
Matricaria chamomilla L. has been used for centuries in many applications, including antiparasitic activity. Leishmaniasis is a parasitic disease, with limited treatments, due to high cost and toxicity. Thus, there is a need to develop new treatments, and in this context, natural products are targets of these researches. We report the development of chitosan nanocapsules containing essential oil of M. chamomilla (CEO) from oil-in-water emulsions using chitosan modified with tetradecyl chains as biocompatible shell material. The nanocapsules of CEO (NCEO) were analyzed by optical microscopy and dynamic light scattering, which revealed spherical shape and an average size of 800 nm. Successful encapsulation of CEO was further confirmed by fluorescence microscopy observations taking advantage of the autofluorescence properties of CEO. The encapsulation efficiency was around 90%. The entrapment of CEO reduced its cytotoxicity towards normal cells. On the other hand, the CEO was active against promastigotes and intracellular amastigotes, exhibiting IC50 of 3.33 µg/mL and 14.56 µg/mL, respectively, while NCEO showed IC50 for promastigotes of 7.18 µg/mL and for intracellular amastigotes of 14.29 µg/mL. These results demonstrate that encapsulation of CEO in nanocapsules using an alkylated chitosan biosurfactant as a "green" stabilizer is a promising therapeutic strategy to treat leishmaniasis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óleos Voláteis / Leishmaniose Cutânea / Matricaria / Quitosana / Nanocápsulas / Leishmania / Anti-Infecciosos Limite: Humans Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óleos Voláteis / Leishmaniose Cutânea / Matricaria / Quitosana / Nanocápsulas / Leishmania / Anti-Infecciosos Limite: Humans Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda