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Improvement of Aglycone Content in Soy Isoflavones Extract by Free and Immobilized Β-Glucosidase and their Effects in Lipid Accumulation.
Angelotti, Joelise A F; Dias, Fernanda F G; Sato, Hélia H; Fernandes, Pedro; Nakajima, Vânia M; Macedo, Juliana.
Afiliação
  • Angelotti JAF; Institute of Chemistry, Federal University of Alfenas, R. Gabriel Monteiro da Silva, 700, Centro, Alfenas, Minas Gerais, Brazil. joelise.angelotti@unifal-mg.edu.br.
  • Dias FFG; Department of Food Science and Technology, University of California, 2212 Robert Mondavi Institute-South, Davis, CA, 95616, USA.
  • Sato HH; Department of Food Science, Faculty of Food Engineering, State University of Campinas-UNICAMP, Monteiro Lobato, 80, Cidade Universitária, CEP, Campinas, SP, 13083-862, Brazil.
  • Fernandes P; Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001, Lisbon, Portugal.
  • Nakajima VM; DREAMS e Faculdade de Engenharia, Universidade Lusófona de Humanidades e Tecnologias, Av. Campo Grande 376, 1749-024, Lisbon, Portugal.
  • Macedo J; Department of Nutrition and Dietetics, Faculty of Nutrition, Fluminense Federal University-UFVF, rua Mários Santos Braga 30, CEP, Niterói, RJ, 24020-140, Brazil.
Appl Biochem Biotechnol ; 192(3): 734-750, 2020 Nov.
Article em En | MEDLINE | ID: mdl-32535816
Soybean is one of the most important commodities in the world, being applied in feed crops and food, pharmaceutical industries in different ways. Soy is rich in isoflavones that in aglycone forms have exhibited significant anti-obesity and anti-lipogenic effects. Obesity is a global problem as several diseases have been related to this worldwide epidemic. The aim of this work was to verify the effect of free and immobilized ß-glucosidase, testing Lentikats, and sol-gel as carriers. Moreover, we wanted to examine if the different types of hydrolysis would generate extracts with distinct biological activity concerning lipid accumulation, PPAR-α regulation, and TNF-α, IL-6, and IL-10 concentrations using in vitro assays. Our results show that all formulations of ß-glucosidase could hydrolyze soy isoflavones. Thus, after 24 h of incubation, daidzein content increased 2.6-, 10.8-, and 12.2-fold; and genistein content increased 11.7, 11.4, and 11.4 times with the use of free enzyme, Lentikats®, and sol-gel immobilized enzyme, respectively. Moreover, both methodologies for enzyme immobilization led to promising forms of biocatalysts for application in the production of soy extracts rich in isoflavones aglycones, which are expected to bring about health benefits. A mild lipogenic effect was observed for some concentrations of extracts, as well as a slight inhibition in PPAR-α expression, although no significant differences were noticeable in the cytokines TNF-α, IL-10, and IL-6 as compared with the control.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glycine max / Beta-Glucosidase / Iridoides / Enzimas Imobilizadas / Metabolismo dos Lipídeos / Isoflavonas Limite: Animals Idioma: En Revista: Appl Biochem Biotechnol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glycine max / Beta-Glucosidase / Iridoides / Enzimas Imobilizadas / Metabolismo dos Lipídeos / Isoflavonas Limite: Animals Idioma: En Revista: Appl Biochem Biotechnol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos