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Nisin and acid resistance in Salmonella is enhanced by N-dodecanoyl-homoserine lactone.
Luiz de Freitas, Leonardo; Aparecida Dos Santos, Clarissa Isabela; Carneiro, Deisy Guimarães; Dantas Vanetti, Maria Cristina.
Afiliação
  • Luiz de Freitas L; Department of Microbiology, Universidade Federal de Viçosa (UFV), Av. Peter Henry Rolfs, S/N, Viçosa, MG, Brazil.
  • Aparecida Dos Santos CI; Department of Microbiology, Universidade Federal de Viçosa (UFV), Av. Peter Henry Rolfs, S/N, Viçosa, MG, Brazil.
  • Carneiro DG; Department of Microbiology, Universidade Federal de Viçosa (UFV), Av. Peter Henry Rolfs, S/N, Viçosa, MG, Brazil.
  • Dantas Vanetti MC; Department of Microbiology, Universidade Federal de Viçosa (UFV), Av. Peter Henry Rolfs, S/N, Viçosa, MG, Brazil. Electronic address: mvanetti@ufv.br.
Microb Pathog ; 147: 104320, 2020 Oct.
Article em En | MEDLINE | ID: mdl-32534181
Salmonella is a foodborne pathogen that can develop resistance to different stresses, which is essential for successful infection of the host. Some genes directly related to acid resistance are also involved in cationic peptide resistance in Gram-negative bacteria and could be under the control of quorum sensing (QS) mediated by autoinducer 1, known as acyl-homoserine lactone. Here, we investigated the influence of autoinducer 1, N-dodecanoyl-homoserine lactone (C12-HSL) on the resistance of Salmonella enterica subspecies enterica serovar Enteritidis to nisin and acid stress. Salmonella cells growing in anaerobic tryptic soy agar (TSB) at a pH of 7.0 for 7 h were submitted to acid stress at a pH of 4.5 in the presence and absence of nisin and were either supplemented or not with C12-HSL. Viable cell counts, gene expression, membrane charge alterations, fatty acid composition, and intracellular content leakage were observed. The autoinducer C12-HSL increased nisin resistance and survival at a pH of 4.5 in Salmonella. Also, C12-HSL increased the expression of the genes, phoP, phoQ, pmrA, and pmrB, which are involved with antimicrobial and acid resistance. The positive charge on the cell surface and concentration of cyclopropane fatty acid of the cellular membrane were increased in the presence of C12-HSL under acidic conditions, whereas membrane fluidity decreased. The loss of K+ and NADPH, promoted by nisin, was reduced in the presence of C12-HSL at a pH of 4.5. Taken together, these findings suggest that quorum sensing plays an important role in enhanced nisin and acid resistance in Salmonella.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Homosserina / Nisina Idioma: En Revista: Microb Pathog Assunto da revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Homosserina / Nisina Idioma: En Revista: Microb Pathog Assunto da revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido