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Identification of cross-reactive human single-chain variable fragments against phospholipases A2 from Lachesis muta and Bothrops spp venoms.
Campos, Lucas B; Pucca, Manuela B; Silva, Luciano C; Pessenda, Gabriela; Filardi, Bruno A; Cerni, Felipe A; Oliveira, Isadora S; Laustsen, Andreas H; Arantes, Eliane C; Barbosa, José E.
Afiliação
  • Campos LB; Department of Biochemistry and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
  • Pucca MB; Medical School, Federal University of Roraima, Boa Vista, RR, Brazil; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark. Electronic address: manu.pucca@ufrr.br.
  • Silva LC; Department of Biochemistry and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil; Toronto Recombinant Antibody Centre, The Donnelly Centre, University of Toronto, Toronto, Canada.
  • Pessenda G; Department of Biochemistry and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
  • Filardi BA; Department of Biochemistry and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
  • Cerni FA; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark; Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
  • Oliveira IS; Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
  • Laustsen AH; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark.
  • Arantes EC; Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
  • Barbosa JE; Department of Biochemistry and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Toxicon ; 184: 116-121, 2020 Sep.
Article em En | MEDLINE | ID: mdl-32505638
Bushmasters (Lachesis spp) and lancehead vipers (Bothrops spp) are two of the most dangerous snakes found in Latin America. Victims of envenoming by these snakes require urgent administration of antivenom. Here, we report the identification of a small set of broadly neutralizing human monoclonal single-chain variable fragment (scFv) antibodies targeting key phospholipases A2 from Lachesis and Bothrops spp using phage display technology and demonstrate their in vitro efficacy using a hemolysis assay.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Viperidae / Venenos de Crotalídeos / Anticorpos de Cadeia Única Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Toxicon Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Viperidae / Venenos de Crotalídeos / Anticorpos de Cadeia Única Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Toxicon Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido