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miR-927 has pro-viral effects during acute and persistent infection with dengue virus type 2 in C6/36 mosquito cells.
Avila-Bonilla, Rodolfo Gamaliel; Yocupicio-Monroy, Martha; Marchat, Laurence A; Pérez-Ishiwara, David Guillermo; Cerecedo-Mercado, Doris Atenea; Del Ángel, Rosa María; Salas-Benito, Juan Santiago.
Afiliação
  • Avila-Bonilla RG; Programa de Doctorado en Ciencias en Biotecnología, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Guillermo Massieu Helguera 249, La Escalera-Ticomán, Mexico City CP 07320, Mexico.
  • Yocupicio-Monroy M; Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México, San Lorenzo 290, Del Valle, Mexico City CP 03100, Mexico.
  • Marchat LA; Programa Institucional de Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Guillermo Massieu Helguera 249, La Escalera-Ticomán, Mexico City CP 07320, Mexico.
  • Pérez-Ishiwara DG; Programa de Doctorado en Ciencias en Biotecnología, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Guillermo Massieu Helguera 249, La Escalera-Ticomán, Mexico City CP 07320, Mexico.
  • Cerecedo-Mercado DA; Programa Institucional de Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Guillermo Massieu Helguera 249, La Escalera-Ticomán, Mexico City CP 07320, Mexico.
  • Del Ángel RM; Programa de Doctorado en Ciencias en Biotecnología, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Guillermo Massieu Helguera 249, La Escalera-Ticomán, Mexico City CP 07320, Mexico.
  • Salas-Benito JS; Programa Institucional de Biomedicina Molecular, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Guillermo Massieu Helguera 249, La Escalera-Ticomán, Mexico City CP 07320, Mexico.
J Gen Virol ; 101(8): 825-839, 2020 08.
Article em En | MEDLINE | ID: mdl-32478656
Dengue virus (DENV) is an important flavivirus that is transmitted to humans by Aedes mosquitoes, where it can establish a persistent infection underlying vertical and horizontal transmission. However, the exact mechanism of persistent DENV infection is not well understood. Recently miR-927 was found to be upregulated in C6/36-HT cells at 57 weeks of persistent infection (C6-L57), suggesting its participation during this type of infection. The aim of this study was to determine the role of miR-927 during infection with DENV type 2. The results indicate an overexpression of miR-927 in C6-L57 cells and acutely infected cells according to the time of infection and the m.o.i. used. The downregulation of miR-927 in C6-L57 cells results in a reduction of both viral titre and viral genome copy number. The overexpression of miR-927 in C6-L40 and C6/36 cells infected at an m.o.i. of 0.1 causes an increase in both viral titre and viral genome copy number, suggesting a pro-viral activity of miR-927. In silico prediction analysis reveals target mRNAs for miR-927 are implicated in post-translational modifications (SUMO), translation factors (eIF-2B), the innate immune system (NKIRAS), exocytosis (EXOC-2), endocytosis (APM1) and the cytoskeleton (FLN). The expression levels of FLN were the most affected by both miR-927 overexpression and inhibition, and FLN was determined to be a direct target of miR-927 by a dual-luciferase gene reporter assay. FLN has been associated with the regulation of the Toll pathway and either overexpression or downregulation of miR-927 resulted in expression changes of antimicrobial peptides (Cecropins A and G, and Defensin D) involved in the Toll pathway response.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aedes / Dengue / Vírus da Dengue / MicroRNAs Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Gen Virol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aedes / Dengue / Vírus da Dengue / MicroRNAs Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Gen Virol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido