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Melatonin membrane receptors MT1 and MT2 are expressed in ram spermatozoa from non-seasonal breeds.
Carvajal-Serna, Melissa; Neira-Rivera, Eliana; Cardozo, Jaime Antonio; Grajales-Lombana, Henry; Cebrián-Pérez, José Álvaro; Muiño-Blanco, Teresa; Pérez-Pé, Rosaura; Casao, Adriana.
Afiliação
  • Carvajal-Serna M; Departmento de Producción Animal, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogotá, Colombia.
  • Neira-Rivera E; Grupo BIOFITER, Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Universidad de Zaragoza, C/ Miguel Servet, 177, 50013, Zaragoza, Spain.
  • Cardozo JA; Centro de Investigación -TIBAITATÁ, Corporación colombiana de Investigación Agropecuaria-AGROSAVIA, Mosquera, Colombia.
  • Grajales-Lombana H; Centro de Investigación -TIBAITATÁ, Corporación colombiana de Investigación Agropecuaria-AGROSAVIA, Mosquera, Colombia.
  • Cebrián-Pérez JÁ; Departmento de Producción Animal, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogotá, Colombia.
  • Muiño-Blanco T; Grupo BIOFITER, Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Universidad de Zaragoza, C/ Miguel Servet, 177, 50013, Zaragoza, Spain.
  • Pérez-Pé R; Grupo BIOFITER, Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Universidad de Zaragoza, C/ Miguel Servet, 177, 50013, Zaragoza, Spain.
  • Casao A; Grupo BIOFITER, Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Instituto Universitario de Investigación en Ciencias Ambientales de Aragón (IUCA), Universidad de Zaragoza, C/ Miguel Servet, 177, 50013, Zaragoza, Spain.
Trop Anim Health Prod ; 52(5): 2549-2557, 2020 Sep.
Article em En | MEDLINE | ID: mdl-32445158
In mammals, many melatonin biological functions are mediated through its interaction with the membrane receptors MT1 and MT2. We have previously reported their presence in ram spermatozoa from males located in temperate climates, but there is no information on their presence in spermatozoa from rams in areas with an equatorial photoperiod (12L:12D). Thus, we have investigated the existence and cellular distribution of melatonin receptors in spermatozoa from three sheep breeds in Colombia (Colombian Creole, Hampshire, and Romney Marsh) during dry and rainy seasons, using indirect immunofluorescence and western blot. Our results indicated the presence of melatonin receptors in spermatozoa from these rams, and that their distribution differs from that previously found in spermatozoa from rams in temperate climates. Moreover, two new immunotypes of MT2 were identified: type N, with staining only in the neck, and type E with a band of immunofluorescence in the upper part of the post-acrosome and the apical edge. Likewise, differences between breeds and climate seasons were detected for both receptors. However, densitometry analysis of western blot bands only revealed differences between seasons in the Creole rams for MT1 and the Romney Marsh rams for MT2, whereas differences between breeds were only detected for MT2. It could be inferred that melatonin receptors in rams subjected to an equatorial photoperiod might be more closely related to sperm quality than seasonal control. Therefore, the presence of these receptors suggests that melatonin could be a useful tool to increase the fertility of rams located in tropical or equatorial climates.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ovinos / Receptor MT1 de Melatonina / Receptor MT2 de Melatonina Limite: Animals Idioma: En Revista: Trop Anim Health Prod Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ovinos / Receptor MT1 de Melatonina / Receptor MT2 de Melatonina Limite: Animals Idioma: En Revista: Trop Anim Health Prod Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Estados Unidos