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Crotalaria spectabilis as a source of pyrrolizidine alkaloids and phenolic compounds: HPLC-MS/MS dereplication and monocrotaline quantification of seed and leaf extracts.
Scupinari, Tamires; Mannochio Russo, Helena; Sabino Ferrari, Anna Beatriz; da Silva Bolzani, Vanderlan; Dias, Waldir Pereira; de Oliveira Nunes, Estela; Hoffmann-Campo, Clara Beatriz; Zeraik, Maria Luiza.
Afiliação
  • Scupinari T; Laboratory of Phytochemistry and Biomolecules (LabFitoBio), Department of Chemistry, State University of Londrina (UEL), Londrina, PR, Brazil.
  • Mannochio Russo H; Nuclei of Bioassays, Biosynthesis and Ecophysiology of Natural Products (NuBBE), Department of Organic Chemistry, Institute of Chemistry, São Paulo State University (UNESP), Araraquara, SP, Brazil.
  • Sabino Ferrari AB; Laboratory of Phytochemistry and Biomolecules (LabFitoBio), Department of Chemistry, State University of Londrina (UEL), Londrina, PR, Brazil.
  • da Silva Bolzani V; Nuclei of Bioassays, Biosynthesis and Ecophysiology of Natural Products (NuBBE), Department of Organic Chemistry, Institute of Chemistry, São Paulo State University (UNESP), Araraquara, SP, Brazil.
  • Dias WP; Brazilian Agricultural Research Corporation - Embrapa Soybean, Londrina, PR, Brazil.
  • de Oliveira Nunes E; Brazilian Agricultural Research Corporation - Embrapa Soybean, Londrina, PR, Brazil.
  • Hoffmann-Campo CB; Brazilian Agricultural Research Corporation - Embrapa Soybean, Londrina, PR, Brazil.
  • Zeraik ML; Laboratory of Phytochemistry and Biomolecules (LabFitoBio), Department of Chemistry, State University of Londrina (UEL), Londrina, PR, Brazil.
Phytochem Anal ; 31(6): 747-755, 2020 Nov.
Article em En | MEDLINE | ID: mdl-32428987
INTRODUCTION: Crotalaria spectabilis is an important species used as a pre-plant cover for soybean crops to control the proliferation of endoparasitic nematodes. Species from the Crotalaria genus are known for presenting pyrrolizidine alkaloids (PAs) in their composition, however, C. spectabilis is still considered chemically under-explored. OBJECTIVE: The goal of this manuscript is the development and validation of a method for PAs and flavonoids identification and quantification of C. spectabilis seeds and leaves, a toxic plant used for nematode proliferation control in soil, especially in soybean crops. MATERIALS AND METHODS: Seeds and leaves extracts were analysed by high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) for the identification of the compounds. RESULTS: PAs and phenolic compounds could be identified in both samples based on the MS/MS fragmentation pattern. Molecular formulas of the annotated compounds were confirmed by ultra-high-performace liquid chromatography-quadrupole time-of-flight (UHPLC-QToF), and monocrotaline could also be confirmed by standard comparison. The quantification of monocrotaline was performed by HPLC-MS/MS, resulting in 123 times higher monocrotaline content in seeds than in the leaves, which could explain its efficiency in combating nematode proliferation in soil. CONCLUSION: This was the first report of phenolic compounds in C. spectabilis. The current study highlights the importance of C. spectabilis for nematode control due to the presence of toxic PAs, and the employment of analytical techniques for identification and quantification of compounds present in the extracts.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Alcaloides de Pirrolizidina / Crotalaria Idioma: En Revista: Phytochem Anal Assunto da revista: BOTANICA / QUIMICA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Alcaloides de Pirrolizidina / Crotalaria Idioma: En Revista: Phytochem Anal Assunto da revista: BOTANICA / QUIMICA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido