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Neutrophils activated by BJcuL, a C-type lectin isolated from Bothrops jararacussu venom, decrease the invasion potential of neuroblastoma SK-N-SH cells in vitro.
Carneiro-Goetten, Jessica Ohana Lemes; Rodrigues, Bruna Santos; Nogoceke, Rodrigo Amauri; do Nascimento, Thatyanne Gradowski; Moreno-Amaral, Andrea Novais; Stuelp-Campelo, Patricia Maria; Elifio-Esposito, Selene.
Afiliação
  • Carneiro-Goetten JOL; Programa de Pós-graduação em Ciências da Saúde, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, PR, Brazil.
  • Rodrigues BS; Escola de Ciências da Vida, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, PR, Brazil.
  • Nogoceke RA; Escola de Ciências da Vida, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, PR, Brazil.
  • do Nascimento TG; Programa de Pós-graduação em Ciências da Saúde, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, PR, Brazil.
  • Moreno-Amaral AN; Programa de Pós-graduação em Ciências da Saúde, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, PR, Brazil.
  • Stuelp-Campelo PM; Escola de Ciências da Vida, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, PR, Brazil.
  • Elifio-Esposito S; Escola de Ciências da Vida, Pontifícia Universidade Católica do Paraná (PUCPR), Curitiba, PR, Brazil.
J Venom Anim Toxins Incl Trop Dis ; 26: e20190073, 2020.
Article em En | MEDLINE | ID: mdl-32425992
BACKGROUND: Neuroblastoma is a pediatric tumor with a mortality rate of 40% in the most aggressive cases. Tumor microenvironment components as immune cells contribute to the tumor progression; thereby, the modulation of immune cells to a pro-inflammatory and antitumoral profile could potentialize the immunotherapy, a suggested approach for high-risk patients. Preview studies showed the antitumoral potential of BJcuL, a C- type lectin isolated from Bothrops jararacussu venom. It was able to induce immunomodulatory responses, promoting the rolling and adhesion of leukocytes and the activation of neutrophils. METHODS: SK-N-SH cells were incubated with conditioned media (CM) obtained during the treatment of neutrophils with BJcuL and fMLP, a bacteria-derived peptide highly effective for activating neutrophil functions. Then we evaluated the effect of the same stimulation on the co-cultivation of neutrophils and SK-N-SH cells. Tumor cells were tested for viability, migration, and invasion potential. RESULTS: In the viability assay, only neutrophils treated with BJcuL (24 h) and cultivated with SK-N-SH were cytotoxic. Migration of tumor cells decreased when incubated directly (p < 0.001) or indirectly (p < 0.005) with untreated neutrophils. When invasion potential was evaluated, neutrophils incubated with BJcuL reduced the total number of colonies of SK-N-SH cells following co-cultivation for 24 h (p < 0.005). Treatment with CM resulted in decreased anchorage-free survival following 24 h of treatment (p < 0.001). CONCLUSION: Data demonstrated that SK-N-SH cells maintain their migratory potential in the face of neutrophil modulation by BJcuL, but their invasive capacity was significantly reduced.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: J Venom Anim Toxins Incl Trop Dis Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: J Venom Anim Toxins Incl Trop Dis Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil