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Novel tephritid-specific features revealed from cytological and transcriptomic analysis of Anastrepha ludens embryonic development.
Gutiérrez-Ramos, Ximena; Vázquez, Martha; Dorantes-Acosta, Ana E; Díaz-Fleischer, Francisco; Peralta-Alvarez, Carlos A; Nuñez-Martínez, Hober N; Arzate-Mejía, Rodrigo G; Recillas-Targa, Félix; Arteaga-Vázquez, Mario A; Zurita, Mario.
Afiliação
  • Gutiérrez-Ramos X; Departamento de Genética Del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico; Group of Epigenetics and Developmental Biology, INBIOTECA, Instituto de Biotecnología y Ecología Aplicada, Universidad Veracruzana, Mexico.
  • Vázquez M; Departamento de Genética Del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico.
  • Dorantes-Acosta AE; Group of Epigenetics and Developmental Biology, INBIOTECA, Instituto de Biotecnología y Ecología Aplicada, Universidad Veracruzana, Mexico.
  • Díaz-Fleischer F; Group of Epigenetics and Developmental Biology, INBIOTECA, Instituto de Biotecnología y Ecología Aplicada, Universidad Veracruzana, Mexico.
  • Peralta-Alvarez CA; Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico.
  • Nuñez-Martínez HN; Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico.
  • Arzate-Mejía RG; Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico.
  • Recillas-Targa F; Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico.
  • Arteaga-Vázquez MA; Group of Epigenetics and Developmental Biology, INBIOTECA, Instituto de Biotecnología y Ecología Aplicada, Universidad Veracruzana, Mexico. Electronic address: maarteaga@uv.mx.
  • Zurita M; Departamento de Genética Del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Mexico. Electronic address: marioz@ibt.unam.mx.
Insect Biochem Mol Biol ; 122: 103412, 2020 07.
Article em En | MEDLINE | ID: mdl-32417415
Anastrepha ludens is a major pest of fruits including citrus and mangoes in Mexico and Central America with major economic and social impacts. Despite its importance, our knowledge on its embryonic development is scarce. Here, we report the first cytological study of embryonic development in A. ludens and provide a transcriptional landscape during key embryonic stages. We established 17 stages of A. ludens embryogenesis that closely resemble the morphological events observed in Drosophila. In addition to the extended duration of embryonic development, we observed notable differences including yolk extrusion at both poles of the embryo, distinct nuclear division waves in the syncytial blastoderm and a heterochronic change during the involution of the head. Characterization of the transcriptional dynamics during syncytial blastoderm, cellular blastoderm and gastrulation, showed that approximately 9000 different transcripts are present at each stage. Even though we identified most of the transcripts with a role during embryonic development present in Drosophila, including sex determination genes, a number of transcripts were absent not only in A. ludens but in other tephritids such as Ceratitis capitata and Bactrocera dorsalis. Intriguingly, some A. ludens embryo transcripts encode proteins present in other organisms but not in other flies. Furthermore, we developed an RNA in situ hybridization protocol that allowed us to obtain the expression patterns of genes whose functions are important in establishing the embryonic body pattern. Our results revealed novel tephritid-specific features during A. ludens embryonic development and open new avenues for strategies aiming to control this important pest.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tephritidae / Desenvolvimento Embrionário / Transcriptoma Limite: Animals Idioma: En Revista: Insect Biochem Mol Biol Assunto da revista: BIOLOGIA MOLECULAR / BIOQUIMICA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tephritidae / Desenvolvimento Embrionário / Transcriptoma Limite: Animals Idioma: En Revista: Insect Biochem Mol Biol Assunto da revista: BIOLOGIA MOLECULAR / BIOQUIMICA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido