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Electrochemical detection of Toxocara canis excretory-secretory antigens in children from rural communities in Esmeraldas Province, Ecuador: association between active infection and high eosinophilia.
Morales-Yánez, Francisco; Trashin, Stanislav; Sariego, Idalia; Roucher, Clémentine; Paredis, Linda; Chico, Martha; De Wael, Karolien; Muyldermans, Serge; Cooper, Philip; Polman, Katja.
Afiliação
  • Morales-Yánez F; Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium. Francisco.Morales.Yanez@vub.be.
  • Trashin S; Department of Biomedical Sciences, Unit of Medical Helminthology, Institute of Tropical Medicine, Antwerp, Belgium. Francisco.Morales.Yanez@vub.be.
  • Sariego I; AXES Research Group, Department of Chemistry, University of Antwerp, Antwerp, Belgium.
  • Roucher C; Institute of Tropical Medicine "Pedro Kourí", Havana, Cuba.
  • Paredis L; Department of Biomedical Sciences, Unit of Medical Helminthology, Institute of Tropical Medicine, Antwerp, Belgium.
  • Chico M; Department of Biomedical Sciences, Unit of Medical Helminthology, Institute of Tropical Medicine, Antwerp, Belgium.
  • De Wael K; Fundación Ecuatoriana Para Investigación en Salud, Quito, Ecuador.
  • Muyldermans S; AXES Research Group, Department of Chemistry, University of Antwerp, Antwerp, Belgium.
  • Cooper P; Laboratory of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium.
  • Polman K; Fundación Ecuatoriana Para Investigación en Salud, Quito, Ecuador.
Parasit Vectors ; 13(1): 245, 2020 May 12.
Article em En | MEDLINE | ID: mdl-32398157
BACKGROUND: The diagnosis of active Toxocara canis infections in humans is challenging. Larval stages of T. canis do not replicate in human tissues and disease may result from infection with a single T. canis larva. Recently, we developed a nanobody-based electrochemical magnetosensor assay with superior sensitivity to detect T. canis excretory-secretory (TES) antigens. Here, we evaluate the performance of the assay in children from an Ecuadorian birth cohort that followed children to five years of age. METHODS: Samples were selected based on the presence of peripheral blood eosinophilia and relative eosinophil counts. The samples were analyzed by the nanobody-based electrochemical magnetosensor assay, which utilizes a bivalent biotinylated nanobody as capturing agent on the surface of streptavidin pre-coated paramagnetic beads. Detection was performed by a different nanobody chemically labelled with horseradish peroxidase. RESULTS: Of 87 samples tested, 33 (38%) scored positive for TES antigen recognition by the electrochemical magnetosensor assay. The average concentration of TES antigen in serum was 2.1 ng/ml (SD = 1.1). The positive result in the electrochemical assay was associated with eosinophilia > 19% (P = 0.001). Parasitological data were available for 57 samples. There was no significant association between positivity by the electrochemical assay and the presence of other soil-transmitted helminth infections. CONCLUSIONS: Our nanobody-based electrochemical assay provides highly sensitive quantification of TES antigens in serum and has potential as a valuable tool for the diagnosis of active human toxocariasis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Toxocaríase / Proteínas de Helminto / Eosinofilia / Técnicas Eletroquímicas / Anticorpos de Domínio Único / Antígenos de Helmintos Tipo de estudo: Diagnostic_studies / Risk_factors_studies Limite: Animals / Child, preschool / Humans / Infant País/Região como assunto: America do sul / Ecuador Idioma: En Revista: Parasit Vectors Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Bélgica País de publicação: Reino Unido
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Toxocaríase / Proteínas de Helminto / Eosinofilia / Técnicas Eletroquímicas / Anticorpos de Domínio Único / Antígenos de Helmintos Tipo de estudo: Diagnostic_studies / Risk_factors_studies Limite: Animals / Child, preschool / Humans / Infant País/Região como assunto: America do sul / Ecuador Idioma: En Revista: Parasit Vectors Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Bélgica País de publicação: Reino Unido