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Plasmid gene AZOBR_p60126 impacts biosynthesis of lipopolysaccharide II and swarming motility in Azospirillum brasilense Sp245.
Petrova, Lilia P; Yevstigneyeva, Stella S; Borisov, Igor V; Shelud'ko, Andrei V; Burygin, Gennady L; Katsy, Elena I.
Afiliação
  • Petrova LP; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia.
  • Yevstigneyeva SS; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia.
  • Borisov IV; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia.
  • Shelud'ko AV; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia.
  • Burygin GL; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia.
  • Katsy EI; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia.
J Basic Microbiol ; 60(7): 613-623, 2020 Jul.
Article em En | MEDLINE | ID: mdl-32378235
The facultative plant endophyte Azospirillum brasilense Sp245 synthesizes two high-molecular-weight lipopolysaccharides, LPSI and LPSII, which comprise identical d-rhamnan O-polysaccharides and, presumably different core oligosaccharides. Previously, using random insertion mutagenesis, we constructed the LpsII- mutant KM139 of strain Sp245 that possessed an Omegon-Km insertion in plasmid AZOBR_p6. Here, we found that in KM139, Omegon-Km disrupted the coding sequence AZOBR_p60126 for a putative glycosyltransferase related to mannosyltransferases and rhamnosyltransferases. To verify its function, we cloned the AZOBR_p60126 gene of strain Sp245 in the expression vector plasmid pRK415 and transferred the construct pRK415-p60126 into KM139. In the complemented mutant KM139 (pRK415-p60126), the wild-type LPSI+ LPSII+ profile was recovered. We also compared the swimming and swarming motilities of strains Sp245, Sp245 (pRK415), KM139, KM139 (pRK415), and KM139 (pRK415-p60126). All these strains had the same flagellar-dependent swimming speeds, but on soft media, the LpsI+ LpsII- strains KM139 and KM139 (pRK415) swarmed significantly faster than the other LpsI+ LpsII+ strains. Such interstrain differences in swarming motility were more pronounced on 0.4% than on 0.5% soft agar plates. These data show that the AZOBR_p60126-encoded putative glycosyltransferase significantly affects the lipopolysaccharide profile and, as a consequence, the social motility of azospirilla.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Azospirillum brasilense / Lipopolissacarídeos / Glicosiltransferases / Locomoção País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Basic Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Federação Russa País de publicação: Alemanha
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Azospirillum brasilense / Lipopolissacarídeos / Glicosiltransferases / Locomoção País/Região como assunto: America do sul / Brasil Idioma: En Revista: J Basic Microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Federação Russa País de publicação: Alemanha