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Influence of the Phenological State of in the Antioxidant Potential and Chemical Composition of Ageratina havanensis. Effects on the P-Glycoprotein Function.
García, Trina H; Rocha, Claudia Quintino da; Delgado-Roche, Livan; Rodeiro, Idania; Ávila, Yaiser; Hernández, Ivones; Cuellar, Cindel; Lopes, Miriam Teresa Paz; Vilegas, Wagner; Auriemma, Giulia; Spengler, Iraida; Rastrelli, Luca.
Afiliação
  • García TH; Center for Natural Product Research, Faculty of Chemistry, University of Havana, Havana 10400, Cuba.
  • Rocha CQD; Department of Chemistry, Federal University of Maranhão, São Luis, Maranhão 65080-805, Brazil.
  • Delgado-Roche L; Department of Pharmacology, Institute of Marine Sciences (ICIMAR), Loma 14, Alturas del Vedado, Plaza de la Revolución, Havana 10400, Cuba.
  • Rodeiro I; Department of Pharmacology, Institute of Marine Sciences (ICIMAR), Loma 14, Alturas del Vedado, Plaza de la Revolución, Havana 10400, Cuba.
  • Ávila Y; Institute of Ecology and Systematics, Havana 10400, Cuba.
  • Hernández I; Department of Pharmacology, Institute of Marine Sciences (ICIMAR), Loma 14, Alturas del Vedado, Plaza de la Revolución, Havana 10400, Cuba.
  • Cuellar C; Department of Pharmacology, Institute of Marine Sciences (ICIMAR), Loma 14, Alturas del Vedado, Plaza de la Revolución, Havana 10400, Cuba.
  • Lopes MTP; Laboratory of Antitumor Natural Substances, Department of Pharmacology, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte 31207-90, Brazil.
  • Vilegas W; Experimental Campus of Sao Vicente, UNESP-Sao Paulo State University, Sao Vicente, Sao Paulo 11.330-900, Brazil.
  • Auriemma G; Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 84084 Fisciano (SA), Italy.
  • Spengler I; Center for Natural Product Research, Faculty of Chemistry, University of Havana, Havana 10400, Cuba.
  • Rastrelli L; Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 84084 Fisciano (SA), Italy.
Molecules ; 25(9)2020 May 02.
Article em En | MEDLINE | ID: mdl-32370149
Ageratina havanensis (Kunth) R. M. King & H. Robinson is a species of flowering shrub in the family Asteraceae, native to the Caribbean and Texas. The aim of this work was to compare the quantitative chemical composition of extracts obtained from Ageratina havanensis in its flowering and vegetative stages with the antioxidant potential and to determine the effects on P-glycoprotein (P-gp) function. The quantitative chemical composition of the extracts was determined quantifying their major flavonoids by UPLC-ESI-MS/MS and by PCA analysis. The effects of the extracts on P-gp activity was evaluated by Rhodamine 123 assay; antioxidant properties were determined by DPPH, FRAP and inhibition of lipid peroxidation methods. The obtained results show that major flavonoids were present in higher concentrations in vegetative stage than flowering stage. In particular, the extracts obtained in the flowering season showed a significantly higher ability to sequester free radicals compared to those of the vegetative season, meanwhile, the extracts obtained during the vegetative stage showed a significant inhibitory effect against brain lipid peroxidation and a strong reductive capacity. This study also showed the inhibitory effects of all ethanolic extracts on P-gp function in 4T1 cell line; these effects were unrelated to the phenological stage. This work shows, therefore, the first evidence on: the inhibition of P-gp function, the antioxidant effects and the content of major flavonoids of Ageratina havanensis. According to the obtained results, the species Ageratina havanensis (Kunth) R. M. King & H. Robinson could be a source of new potential inhibitors of drug efflux mediated by P-gp. A special focus on all these aspects must be taking into account for future studies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Ativação do Canal Iônico / Membro 1 da Subfamília B de Cassetes de Ligação de ATP / Ageratina / Antioxidantes Tipo de estudo: Diagnostic_studies Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Cuba País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Extratos Vegetais / Ativação do Canal Iônico / Membro 1 da Subfamília B de Cassetes de Ligação de ATP / Ageratina / Antioxidantes Tipo de estudo: Diagnostic_studies Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Cuba País de publicação: Suíça