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Detection of Mycobacterium avium subsp. paratuberculosis in reproductive tissue and semen of naturally infected rams.
Velázquez-Morales, José Vicente; Santillán-Flores, Marco Antonio; Gallegos-Sánchez, Jaime; Cuca-García, Juan Manuel; Navarro-Maldonado, María Del Carmen; Rojas-Martínez, Reyna Isabel; Cortez-Romero, César.
Afiliação
  • Velázquez-Morales JV; Colegio de Postgraduados, Programa de Ganadería, Montecillo, Texcoco, México.
  • Santillán-Flores MA; Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Centro Nacional de Investigación Disciplinaria en Microbiología Animal, Ciudad de México, México.
  • Gallegos-Sánchez J; Colegio de Postgraduados, Programa de Ganadería, Montecillo, Texcoco, México.
  • Cuca-García JM; Colegio de Postgraduados, Programa de Ganadería, Montecillo, Texcoco, México.
  • Navarro-Maldonado MDC; Universidad Autónoma Metropolitana Unidad Iztapalapa, Departamento de Biología de la Reproducción, División de Ciencias Biológicas y de la Salud, Ciudad de México, México.
  • Rojas-Martínez RI; Colegio de Postgraduados, Programa de Ganadería, Montecillo, Texcoco, México.
  • Cortez-Romero C; Colegio de Postgraduados, Programa de Ganadería, Montecillo, Texcoco, México.
Anim Reprod ; 16(4): 930-937, 2019 Nov 18.
Article em En | MEDLINE | ID: mdl-32368273
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis (PTB), disease that causes a syndrome of bad nutrient absorption, weight loss and eventually death. The intestine is the main target organ where the infection develops; however, there is evidence of infection by MAP in extra-intestine sites of sheep, including mesenteric nodes and semen. The aim of the study was to identify the presence of MAP in reproductive tissue and semen of infected Pelibuey rams in clinical state of PTB. Seven rams were used in clinical PTB state and a non-infected ram by MAP of the Pelibuey breed, confirmed by serology, nPCR and bacteriological culture, with average weight and age of 57.23 ± 1.73 kg and 2.91 ± 0.17 years, respectively. The presence of MAP was identified in different tissue samples: spleen (1/7, 14.3% and 2/7, 28.6%), small intestine (3/7, 42.9% and 4/7, 57.1%) and mesenteric lymph nodes (3/7, 42.9% and 3/7, 42.9%), with nPCR and culture, respectively. It was also identified in epididymis tissue (1/7, 14.3%), Cowper gland (2/7, 28.6%) and prostate (1/7, 14.3%), using nPCR, although without detection in culture. It was identified in testicular tissue in 42.8% (3/7; culture or nPCR technique), but in 28.6% (2/7) with both techniques. Finally, the presence of MAP was identified in 42.9% (3/7) of semen samples with nPCR; however, it was not detected through culture. In conclusion, the presence of MAP was identified in lymphatic, digestive tissue, and semen; the presence of MAP was reported for the first time in epididymis, Cowper gland, prostate and testicles of infected Pelibuey rams.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies Idioma: En Revista: Anim Reprod Ano de publicação: 2019 Tipo de documento: Article País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies Idioma: En Revista: Anim Reprod Ano de publicação: 2019 Tipo de documento: Article País de publicação: Brasil