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Biochemical analysis of cellobiose catabolism in Candida pseudointermedia strains isolated from rotten wood.
Barrilli, Évelyn T; Tadioto, Viviani; Milani, Letícia M; Deoti, Junior R; Fogolari, Odinei; Müller, Caroline; Barros, Katharina O; Rosa, Carlos A; Dos Santos, Angela A; Stambuk, Boris U; Treichel, Helen; Alves, Sérgio L.
Afiliação
  • Barrilli ÉT; Laboratory of Biochemistry and Genetics, Federal University of Fronteira Sul, Campus Chapecó, Rodovia SC 484, Km 2, Bairro Fronteira Sul, Chapecó, SC, 89815-899, Brazil.
  • Tadioto V; Laboratory of Biochemistry and Genetics, Federal University of Fronteira Sul, Campus Chapecó, Rodovia SC 484, Km 2, Bairro Fronteira Sul, Chapecó, SC, 89815-899, Brazil.
  • Milani LM; Laboratory of Biochemistry and Genetics, Federal University of Fronteira Sul, Campus Chapecó, Rodovia SC 484, Km 2, Bairro Fronteira Sul, Chapecó, SC, 89815-899, Brazil.
  • Deoti JR; Laboratory of Biochemistry and Genetics, Federal University of Fronteira Sul, Campus Chapecó, Rodovia SC 484, Km 2, Bairro Fronteira Sul, Chapecó, SC, 89815-899, Brazil.
  • Fogolari O; Laboratory of Biochemistry and Genetics, Federal University of Fronteira Sul, Campus Chapecó, Rodovia SC 484, Km 2, Bairro Fronteira Sul, Chapecó, SC, 89815-899, Brazil.
  • Müller C; Laboratory of Biochemistry and Genetics, Federal University of Fronteira Sul, Campus Chapecó, Rodovia SC 484, Km 2, Bairro Fronteira Sul, Chapecó, SC, 89815-899, Brazil.
  • Barros KO; Department of Microbiology, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
  • Rosa CA; Department of Microbiology, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
  • Dos Santos AA; Laboratory of Biochemistry and Genetics, Federal University of Fronteira Sul, Campus Chapecó, Rodovia SC 484, Km 2, Bairro Fronteira Sul, Chapecó, SC, 89815-899, Brazil.
  • Stambuk BU; Department of Biochemistry, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Treichel H; Department of Biochemistry, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Alves SL; Laboratory of Microbiology and Bioprocesses, Federal University of Fronteira Sul, Campus Erechim, Erechim, RS, Brazil.
Arch Microbiol ; 202(7): 1729-1739, 2020 Sep.
Article em En | MEDLINE | ID: mdl-32328754
We isolated two Candida pseudointermedia strains from the Atlantic rain forest in Brazil, and analyzed cellobiose metabolization in their cells. After growth in cellobiose medium, both strains had high intracellular ß-glucosidase activity [~ 200 U (g cells)-1 for 200 mM cellobiose and ~ 100 U (g cells)-1 for 2 mM pNPßG] and negligible periplasmic cellobiase activity. During batch fermentation, the strain with the best performance consumed all the available cellobiose in the first 18 h of the assay, producing 2.7 g L-1 of ethanol. Kinetics of its cellobiase activity demonstrated a high-affinity hydrolytic system inside cells, with Km of 12.4 mM. Our data suggest that, unlike other fungal species that hydrolyze cellobiose extracellularly, both analyzed strains transport it to the cytoplasm, where it is then hydrolyzed by high-affinity intracellular ß-glucosidases. We believe this study increases the fund of knowledge regarding yeasts from Brazilian microbiomes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Madeira / Candida / Celobiose / Beta-Glucosidase País/Região como assunto: America do sul / Brasil Idioma: En Revista: Arch Microbiol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Madeira / Candida / Celobiose / Beta-Glucosidase País/Região como assunto: America do sul / Brasil Idioma: En Revista: Arch Microbiol Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha