Measuring the Hydrodynamic Radius of Colloidal Quantum Dots by Fluorescence Correlation Spectroscopy.
Methods Mol Biol
; 2135: 85-93, 2020.
Article
em En
| MEDLINE
| ID: mdl-32246329
Colloidal quantum dots (QDs), due to their versatile optoelectronic properties, have been used in life science applications, especially in fluorescence-based techniques, for over two decades. A great variety of QD syntheses and conjugations are available, and tailoring these for the desired application requires a refined structural characterization. Life science applications rely on the interaction of QDs with biostructures; hence, the knowledge of the QD actual size (i.e., its hydrodynamic radius in the medium the experiment is being carried) and the size of their conjugates is paramount. Fluorescence correlation spectroscopy (FCS) is an optical technique that uses fluorophore light emission to measure its hydrodynamic radius, instead of relying on particle light scattering or crystalline structure, making it ideal for studying bioconjugated QDs in suspension. From the fluorescence intensity autocorrelation, FCS measures the diffusion coefficient of systems in a diluted sample and, by obtaining the diffusion coefficient, it is possible to calculate its hydrodynamic radius. In this chapter we describe the main aspects of the FCS technique and how to use it to calculate the hydrodynamic radius of QDs.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Fluorescência
/
Pontos Quânticos
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2020
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Estados Unidos