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Multifunctional hybrid nanoparticles as magnetic delivery systems for siRNA targeting the HER2 gene in breast cancer cells.
Cristofolini, Tatiane; Dalmina, Milene; Sierra, Jelver A; Silva, Adny H; Pasa, André A; Pittella, Frederico; Creczynski-Pasa, Tânia B.
Afiliação
  • Cristofolini T; GEIMM, Department of Pharmaceutical Sciences, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Dalmina M; GEIMM, Department of Pharmaceutical Sciences, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Sierra JA; GEIMM, Department of Pharmaceutical Sciences, Federal University of Santa Catarina, Florianópolis, SC, Brazil; PGMAT, Department of Mechanical Engineering, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Silva AH; Department of Biochemistry, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Pasa AA; LFFS, Department of Physics, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Pittella F; GEIMM, Department of Pharmaceutical Sciences, Federal University of Santa Catarina, Florianópolis, SC, Brazil; Department of Pharmaceutical Sciences, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
  • Creczynski-Pasa TB; GEIMM, Department of Pharmaceutical Sciences, Federal University of Santa Catarina, Florianópolis, SC, Brazil. Electronic address: tania.pasa@ufsc.br.
Mater Sci Eng C Mater Biol Appl ; 109: 110555, 2020 Apr.
Article em En | MEDLINE | ID: mdl-32228895
Breast cancer is a major cause of death among women worldwide. Resistance to conventional therapies has been observed in HER2-positive breast cancer patients, indicating the need for more effective treatments. Small interfering RNA (siRNA) therapy is an attractive strategy against HER2-positive tumors, but its success depends largely on the efficient delivery of agents to target tissues. In this study, we prepared a magnetic hybrid nanostructure composed of iron oxide nanoparticles coated with caffeic acid and stabilized by layers of calcium phosphate and PEG-polyanion block copolymer for incorporation of siRNA. Transmission electron microscopy images showed monodisperse, neutrally charged compact spheres sized <100 nm. Dynamic light scattering and nanoparticle tracking analysis revealed that the nanostructure had an average hydrodynamic diameter of 130 nm. Nanoparticle suspensions remained stable over 42 days of storage at 4 and 25 °C. Unloaded caffeic acid-magnetic calcium phosphate (Caf-MCaP) nanoparticles were not cytotoxic, and loaded nanoparticles were successfully taken up by the HER2-positive breast cancer cell line HCC1954, even more so under magnetic guidance. Nanoparticles escaped endosomal degradation and delivered siRNA into the cytoplasm, inducing HER2 gene silencing.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / Sistemas de Liberação de Medicamentos / Receptor ErbB-2 / RNA Interferente Pequeno / Nanopartículas / Campos Magnéticos Tipo de estudo: Guideline Limite: Animals / Female / Humans Idioma: En Revista: Mater Sci Eng C Mater Biol Appl Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / Sistemas de Liberação de Medicamentos / Receptor ErbB-2 / RNA Interferente Pequeno / Nanopartículas / Campos Magnéticos Tipo de estudo: Guideline Limite: Animals / Female / Humans Idioma: En Revista: Mater Sci Eng C Mater Biol Appl Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda