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Development of Streptococcus mutans biofilm in the presence of human colostrum and 3'-sialyllactose.
Faria, Juliana Barbosa; Santiago, Marcela Beraldo; Silva, Camilla Beatriz; Geraldo-Martins, Vinicius R; Nogueira, Ruchele Dias.
Afiliação
  • Faria JB; Department of Dentistry, Uberaba University, Uberaba, Minas Gerais, Brazil.
  • Santiago MB; Department of Dentistry, Uberaba University, Uberaba, Minas Gerais, Brazil.
  • Silva CB; Department of Dentistry, Uberaba University, Uberaba, Minas Gerais, Brazil.
  • Geraldo-Martins VR; Department of Dentistry, Uberaba University, Uberaba, Minas Gerais, Brazil.
  • Nogueira RD; Department of Dentistry, Uberaba University, Uberaba, Minas Gerais, Brazil.
J Matern Fetal Neonatal Med ; 35(4): 630-635, 2022 Feb.
Article em En | MEDLINE | ID: mdl-32164475
AIM: To evaluate the initial adhesion and formation of Streptococcus mutans biofilm in vitro in the presence of saliva, human colostrum and 3'-sialyllactose. METHODS: Human colostrum and salivas were collected from 30 mothers and newborn postpartum. Eighteen hours culture of S. mutans was treated with colostrum or 3'-sialyllactose in three different moments: before, during, and after 24 h from the microbial inoculation. Salivas were also tested in conjunction with colostrum. The assays were realized in sterile 96-well flat-bottom microtiter plates for 24 h. The biofilms were fixed, washed, stained with crystal violet, and extracted. Absorbance was measured to evaluate biofilm growth mass. RESULTS: Colostrum applied after and during the inoculation decreased biofilm formation when compared with the control (p < .05). The presence of saliva increased the biofilm biomass (p < .05). The application of 3'-sialyllactose reduced biofilm formation independently of moments of application (p < .05). CONCLUSION: Saliva contributed to the proliferation of biofilm and colostrum did not prevent the initial adhesion, but interfered in the accumulation and development of microorganisms in biofilms. 3'-sialyllactose significantly decreased biofilm formation. This information expands the importance of colostrum as a potent oral antimicrobial biofluid.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Streptococcus mutans / Colostro Limite: Female / Humans / Newborn / Pregnancy Idioma: En Revista: J Matern Fetal Neonatal Med Assunto da revista: OBSTETRICIA / PERINATOLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Streptococcus mutans / Colostro Limite: Female / Humans / Newborn / Pregnancy Idioma: En Revista: J Matern Fetal Neonatal Med Assunto da revista: OBSTETRICIA / PERINATOLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido