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Effect of CPoint, EndoSequence BC, and Gutta-percha Points on Viability and Gene Expression of Periodontal Ligament Fibroblasts.
Meneses, Claudia Bosio; Gambini, Alessandra Fonseca; Olivi, Lucas Tofanello; Santos, Marcelo Dos; Sipert, Carla Renata.
Afiliação
  • Meneses CB; Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, Brazil.
  • Gambini AF; Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, Brazil.
  • Olivi LT; Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, Brazil.
  • Santos MD; Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, Brazil.
  • Sipert CR; Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, Brazil.
Eur Endod J ; 4(2): 57-61, 2019.
Article em En | MEDLINE | ID: mdl-32161888
OBJECTIVE: This study aimed to investigate the cytotoxic and biomodulatory potential of conventional gutta-percha (CGP) points, gutta-percha points containing bioceramics (BC), and CPoint polymer (CP) points on periodontal ligament (PDL) cells in vitro. METHODS: PDL fibroblasts were cultured and stimulated with extracts of CGP, BC, and CP in serial dilutions to evaluate cell viability using MTT assay. Next, the 1:5 dilution was used to stimulate the cells for 72 h to assess the gene expression of type I collagen (COL-1) and cement protein 1 (CEMP-1), by reverse transcription followed by quantitative PCR. Data were statistically analyzed using one-way analysis of variance (ANOVA) (P<0.05). RESULTS: Pure extracts of CGP and CP were found to be cytotoxic for PDL (P<0.01). Once diluted to 1:5, only CP showed cytotoxicity. BC did not affect cell viability in any extract sample. No extract significantly altered the gene expression of COL-1. For CEMP-1, a significant increase in gene expression was observed only for CGP (P<0.05). CONCLUSION: CP was found to be more cytotoxic than CGP, while BC demonstrated no cytotoxicity. The tested cones did not affect COL-1 gene expression, while CGP upregulated CEMP-1. Our results suggest that obturation point components may affect the biological responses of PDL fibroblasts.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Eur Endod J Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Turquia
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Eur Endod J Ano de publicação: 2019 Tipo de documento: Article País de afiliação: Brasil País de publicação: Turquia