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Occurrence of ß-lactam-resistant Staphylococcus aureus in milk from primiparous dairy cows in the northeastern region of Brazil.
Silva, Amanda T F; da Silva, José G; Aragão, Breno B; Peixoto, Rodolfo M; Mota, Rinaldo A.
Afiliação
  • Silva ATF; Universidade Federal Rural de Pernambuco, Av. Manuel de Medeiros, s/n, Dois Irmãos, Recife, PE, 52171-900, Brazil. amanda.tfs@gmail.com.
  • da Silva JG; Universidade Federal Rural de Pernambuco, Av. Manuel de Medeiros, s/n, Dois Irmãos, Recife, PE, 52171-900, Brazil.
  • Aragão BB; Universidade Federal Rural de Pernambuco, Av. Manuel de Medeiros, s/n, Dois Irmãos, Recife, PE, 52171-900, Brazil.
  • Peixoto RM; Instituto Federal de Educação, Ciência e Tecnologia do Sertão Pernambucano, R. Maria Luzia de Araújo, 791, João de Deus, Petrolina, PE, 56316-686, Brazil.
  • Mota RA; Universidade Federal Rural de Pernambuco, Av. Manuel de Medeiros, s/n, Dois Irmãos, Recife, PE, 52171-900, Brazil.
Trop Anim Health Prod ; 52(5): 2303-2307, 2020 Sep.
Article em En | MEDLINE | ID: mdl-32146685
The objective of the current study was to isolate and identify Staphylococcus (S.) aureus strains resistant to beta-lactam antibiotics from primiparous cows' milk. A total of 432 milk samples were collected from all primiparous dairy cows in early lactation that originated from 9 dairy properties. All samples were cultured in Mannitol salt agar enriched with egg yolk emulsion. Determination of genotypic resistance of S. aureus was achieved by polymerase chain reaction (PCR) for amplification of the blaZ, mecA, and mecC genes. Phenotypic resistance of S. aureus strains was evaluated by minimal inhibitory concentration (MIC) technique using broth microdilutions of penicillin G and oxacillin. From all the mammary quarters examined, S. aureus strains were detected in 27 out of 432 (6.25%) milk samples (CI95%, 4.33-8.84). From all dairy properties visited, only two out of 9 were found to have S. aureus. Hence, it was possible to evaluate genotypic and phenotypic resistance in 27 samples from two dairy farms. The isolates of S. aureus had a frequency of (20/27) 74.07% to blaZ gene (CI95%, 57.5-90.6), whereas mecA and mecC genes were not observed. According to MIC results, penicillin G had a 74.07% (20/27) resistance rate (CI95%, 57.5-90.6) and oxacillin had a 14.81% (4/27) resistance rate (CI95%, 1.4-28.2). Thus, the circulation of S. aureus strains resistant to beta-lactams has been confirmed in primiparous dairy cows in the northeastern region of Brazil, indicating the need for new management strategies involving the use of beta-lactam drugs to treat mastitis, discouraging and/or limiting their use. Also, it is important to highlight the need for further studies on epidemiology and traceability of the pathogen.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Estafilocócicas / Staphylococcus aureus / Leite / Beta-Lactamas / Mastite Bovina Limite: Animals / Pregnancy País/Região como assunto: America do sul / Brasil Idioma: En Revista: Trop Anim Health Prod Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções Estafilocócicas / Staphylococcus aureus / Leite / Beta-Lactamas / Mastite Bovina Limite: Animals / Pregnancy País/Região como assunto: America do sul / Brasil Idioma: En Revista: Trop Anim Health Prod Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos