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A durable murine model of spleen transplantation with arterial and venous anastomoses.
Figueiredo, Jose-Luiz; Santa-Cruz, Fernando; Lima-Filho, José Luiz; Hilgendorf, Ingo; Aikawa, Masanori; Pittet, Mikael J; Nahrendorf, Matthias; Weissleder, Ralph; Swirski, Filip K; Robbins, Clinton S.
Afiliação
  • Figueiredo JL; Department of Surgery, Experimental Surgery Unit, Federal University of Pernambuco, Recife, Pernambuco, Brazil. jose.lfigueiredo@ufpe.br.
  • Santa-Cruz F; Medical School, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
  • Lima-Filho JL; Laboratório de Imunopatologia Keizo Asami (LIKA), Federal University of Pernambuco, Recife, Pernambuco, Brazil.
  • Hilgendorf I; Department of Cardiology and Angiology I, Heart Center, University of Freiburg, Freiburg, Germany.
  • Aikawa M; Cardiovascular Division, Brigham and Women's Hospital, Harvard University, Boston, Massachusetts, USA.
  • Pittet MJ; Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
  • Nahrendorf M; Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
  • Weissleder R; Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
  • Swirski FK; Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA.
  • Robbins CS; Department of Radiology, Massachusetts General Hospital, Boston, Massachusetts, USA.
Sci Rep ; 10(1): 3979, 2020 03 04.
Article em En | MEDLINE | ID: mdl-32132617
The spleen is a large lymphoid organ located in the abdomen that filters blood and regulates the immune system. The extent of mobilization of splenic immune cells to peripheral tissues in health and disease, however, remains poorly understood. This is due, in large part, to a lack of in vivo, spleen-specific lineage tagging strategies. Here, we describe a detailed practical protocol of spleen transplantation and its evaluation for long-term graft survival. Unlike implantation of splenic morsels in the great omentum, our approach uses arterial and venous anastomoses which rapidly restores blood flow and facilitates long-term survival of the graft. The use of congenic mouse strains permits the use of immunofluorescence and flow cytometry-based methodologies to unambiguously track the migration of spleen-derived cells to peripheral tissues.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Artérias / Baço / Transplante / Veias / Anastomose Cirúrgica Tipo de estudo: Guideline Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Artérias / Baço / Transplante / Veias / Anastomose Cirúrgica Tipo de estudo: Guideline Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido