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Photobiomodulation therapy improves human dental pulp stem cell viability and migration in vitro associated to upregulation of histone acetylation.
Zaccara, Ivana M; Mestieri, Letícia B; Pilar, Emily F S; Moreira, Maria S; Grecca, Fabiana S; Martins, Manoela D; Kopper, Patrícia Maria Poli.
Afiliação
  • Zaccara IM; Dentistry Graduate Program, Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul - UFRGS, Rua Ramiro Barcelos, 2492, Porto Alegre, Rio Grande do Sul, 90035-003, Brazil.
  • Mestieri LB; Dentistry Graduate Program, Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul - UFRGS, Rua Ramiro Barcelos, 2492, Porto Alegre, Rio Grande do Sul, 90035-003, Brazil.
  • Pilar EFS; Department of Experimental Pathology, Clinics Hospital of Porto Alegre, Federal University of Rio Grande do Sul - UFRGS, Porto Alegre, Rio Grande do Sul, Brazil.
  • Moreira MS; Department of Dentistry, School of Dentistry, University of Sao Paulo, Sao Paulo, Brazil.
  • Grecca FS; Ibirapuera University, Sao Paulo, Brazil.
  • Martins MD; Dentistry Graduate Program, Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul - UFRGS, Rua Ramiro Barcelos, 2492, Porto Alegre, Rio Grande do Sul, 90035-003, Brazil.
  • Kopper PMP; Dentistry Graduate Program, Department of Conservative Dentistry, School of Dentistry, Federal University of Rio Grande do Sul - UFRGS, Rua Ramiro Barcelos, 2492, Porto Alegre, Rio Grande do Sul, 90035-003, Brazil.
Lasers Med Sci ; 35(3): 741-749, 2020 Apr.
Article em En | MEDLINE | ID: mdl-32095920
This in vitro study evaluated the role of photobiomodulation therapy (PBMT) on viability and migration of human dental pulp stem cells (hDPSCs) and its association to epigenetic mechanisms such as histone acetylation. The hDPSCs were characterized and assigned into control and PBMT groups. For the PBMT, five laser irradiations at 6-h intervals were performed using a continuous-wave InGaAlP diode laser. Viability (MTT), migration (scratch), and histone acetylation H3 (H3K9ac immunofluorescence) were evaluated immediately after the last irradiation. PBMT significantly increased the viability (P = 0.004). Also, PBMT group showed significantly increased migration of cells in the wound compared to the control in 6 h (P = 0.002), 12 h (P = 0.014) and 18 h (P = 0.083) being faster than the control, which only finished the process at 24 h. PBMT induced epigenetic modifications in hDPSC due to increased histone acetylation (P = 0.001). PBMT increased viability and migration of hDPSCs, which are related with the upregulation of histone acetylation and could be considered a promising adjuvant therapy for regenerative endodontic treatment.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco / Histonas / Regulação para Cima / Movimento Celular / Terapia com Luz de Baixa Intensidade / Polpa Dentária Tipo de estudo: Risk_factors_studies Limite: Humans Idioma: En Revista: Lasers Med Sci Assunto da revista: BIOTECNOLOGIA / RADIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco / Histonas / Regulação para Cima / Movimento Celular / Terapia com Luz de Baixa Intensidade / Polpa Dentária Tipo de estudo: Risk_factors_studies Limite: Humans Idioma: En Revista: Lasers Med Sci Assunto da revista: BIOTECNOLOGIA / RADIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido