Your browser doesn't support javascript.
loading
New set of microsatellite markers for the Great-billed Seed-finch (Sporophila maximiliani - Passeriformes: Thraupidae): tools for inspection and conservation.
de Melo, Amanda A; Kalapothakis, Evanguedes; Ludwig, Sandra; Baptista, Luiz Alfredo M L; Lara, Mariana P; Silva, Leo C F; Telles, Mariana P de C.
Afiliação
  • de Melo AA; Programa de Pós-Graduação em Genética e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
  • Kalapothakis E; Departamento de Genética, Ecologia e Evolução, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Ludwig S; Departamento de Genética, Ecologia e Evolução, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Baptista LAML; Centro de Triagem de Animais Silvestres de Goiás (CETAS/GO - IBAMA), Goiânia, Goiás, Brazil.
  • Lara MP; Centro de Triagem de Animais Silvestres de Goiás (CETAS/GO - IBAMA), Goiânia, Goiás, Brazil.
  • Silva LCF; Centro de Triagem de Animais Silvestres de Goiás (CETAS/GO - IBAMA), Goiânia, Goiás, Brazil.
  • Telles MPC; Programa de Pós-Graduação em Genética e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Goiás, Brazil. tellesmpc@gmail.com.
Mol Biol Rep ; 47(4): 2997-3002, 2020 Apr.
Article em En | MEDLINE | ID: mdl-32076997
The Thraupidae family is one of the most wanted by bird breeders in Brazil because it is represented by its diverse, colorful and melodious singers. The Great-billed Seed-finch, Sporophila maximiliani, is the only representative of the genus Sporophila considered critically endangered in Brazil. Due to the demands of environmental agencies and of conservation programs, there is a need to increase the number of molecular markers available for the genus and specially for S. maximiliani. Therefore, this work aimed to provide a new set of microsatellite markers for S. maximiliani in order to help bird breeders and environmental agencies on fulfilling its demands as well as contributing with extra genetics tools for conservation programs of the S. maximiliani. Of the 30 markers developed, 25 successfully amplified, and 22 were polymorphic. Annealing temperature varied from 52 to 64 °C, number of alleles from 2 to 13, and the medium allele richness was 7.25 and medium expected, observed heterozygosity and PIC were, respectively, 0.812, 0.661 and 0.752. The probability of identity estimate was 8.54 × 10-27 and all the other probabilities of non-exclusion (sib-identity, parent pair and first-parent) were < 0.001, indicating that this set of microsatellite markers have high genetic variability and high power of individual genetic differentiation for S. maximiliani. Therefore, this work increases the options of molecular markers to be used on inspection for environmental agencies and for conservation programs on analyzing genetic variability and population studies for S. maximiliani.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Repetições de Microssatélites / Tentilhões Limite: Animals Idioma: En Revista: Mol Biol Rep Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Repetições de Microssatélites / Tentilhões Limite: Animals Idioma: En Revista: Mol Biol Rep Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda