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Effect of ScLL and 15d-PGJ2 on viability and cytokine release in LPS-stimulated fibroblasts: an in vitro study.
Reis, Manuella Verdinelli de Paula; Souza, Gabriela Leite de; Soares, Priscilla Barbosa Ferreira; Souza, Maria Aparecida de; Soares, Carlos José; Moura, Camilla Christian Gomes.
Afiliação
  • Reis MVP; Universidade Federal de Uberlândia - UFU, School of Dentistry, Department of Operative Dentistry and Dental Materials, Uberlândia, MG, Brazil.
  • Souza GL; Universidade Federal de Uberlândia - UFU, School of Dentistry, Department of Endodontics, Uberlândia, MG, Brazil.
  • Soares PBF; Universidade Federal de Uberlândia - UFU, School of Dentistry, Department of Oral and Maxillofacial Surgery and Implantology, Uberlândia, MG, Brazil.
  • Souza MA; Universidade Federal de Uberlândia - UFU, Institute of Biomedical Sciences, Department of Immunology, Uberlândia, MG, Brazil.
  • Soares CJ; Universidade Federal de Uberlândia - UFU, School of Dentistry, Department of Operative Dentistry and Dental Materials, Uberlândia, MG, Brazil.
  • Moura CCG; Universidade Federal de Uberlândia - UFU, School of Dentistry, Department of Endodontics, Uberlândia, MG, Brazil.
Braz Oral Res ; 34: e013, 2020.
Article em En | MEDLINE | ID: mdl-32074213
This study evaluated the effect of a cyclopentenone-type PG, 15-Deoxy-Δ12,14-PG J2 (15d-PGJ2), and lectin (ScLL) on the viability of human gingival fibroblasts (HGFs), and on IL-6 and TGFß-1 release by these fibroblasts, stimulated with lipopolysaccharide (LPS). HGFs were stimulated with LPS 10 µg/ml and treated with 15d-PGJ2 1 and 2 µg/ml, and ScLL 2 and 5 µg/ml, for 1 and 3h, and then evaluated for viability by MTT assay. Supernatant was collected to detect IL-6 and TGFß-1 release, by ELISA. Positive control was cells kept in Dulbecco's Modified Eagle's Medium, and negative control was those kept in LPS. Data were analyzed by ANOVA and Dunnett's test (α = 0.05). No significant difference was found in viability among experimental groups at 1h (p > 0.05). Percentage of ScLL 5 µg/ml viable cells was similar to that of positive control at evaluated periods (p > 0.05), whereas the other groups had lower levels than the positive control (p < 0.05). IL-6 release was statistically higher for ScLL 5 µg/ml and 15d-PGJ2 2 µg/ml at 1h, compared with the other treated groups and positive control (p < 0.05). No significant differences were found among the groups at 3h (p > 0.05), except for ScLL 2 µg/ml and 15d-PGJ2 1 µg/ml, which showed lower IL-6 release compared with that of negative control (p < 0.05). No significant difference was found among the groups for TGFß-1 release (p > 0.05). Results indicated that ScLL 5 µg/ml did not interfere in viability, and ScLL 2 µg/ml and 15d-PGJ2 1 µg/ml demonstrated reduced IL-6 release. Tested substances had no effect on TGFß-1 release.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Prostaglandina D2 / Lipopolissacarídeos / Interleucina-6 / Lectinas de Plantas / Fator de Crescimento Transformador beta1 / Fibroblastos Tipo de estudo: Evaluation_studies Limite: Humans Idioma: En Revista: Braz Oral Res Assunto da revista: ODONTOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Prostaglandina D2 / Lipopolissacarídeos / Interleucina-6 / Lectinas de Plantas / Fator de Crescimento Transformador beta1 / Fibroblastos Tipo de estudo: Evaluation_studies Limite: Humans Idioma: En Revista: Braz Oral Res Assunto da revista: ODONTOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil