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Candidemia Candida albicans clusters have higher tendency to form biofilms than singleton genotypes†.
Díaz-García, Judith; Arendrup, Maiken C; Cantón, Rafael; García-Rodríguez, Julio; Gómez, Ana; Gómez, Elia; Orden, Beatriz; Parisi, Gabriella; Pemán, Javier; Posteraro, Brunella; Sanguinetti, Maurizio; Da Matta, Daniel Archimedes; Colombo, Arnaldo L; Muñoz, Patricia; Sánchez-Carrillo, Carlos; Guinea, Jesús; Escribano, Pilar.
Afiliação
  • Díaz-García J; Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
  • Arendrup MC; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
  • Cantón R; Unit of Mycology Statens Serum Institut, Copenhagen, Denmark.
  • García-Rodríguez J; Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
  • Gómez A; Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark.
  • Gómez E; Servicio de Microbiología. Hospital Ramón y Cajal, Madrid and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.
  • Orden B; Red Española de Investigación en Patología Infecciosa (REIPI), Instituto de Salud Carlos III, Madrid, Spain.
  • Parisi G; Clinical Microbiology Department, Hospital La Paz, Madrid, Spain.
  • Pemán J; Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
  • Posteraro B; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
  • Sanguinetti M; Servicio de Microbiología. Hospital Ramón y Cajal, Madrid and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.
  • Da Matta DA; Red Española de Investigación en Patología Infecciosa (REIPI), Instituto de Salud Carlos III, Madrid, Spain.
  • Colombo AL; Hospital Puerta del Hierro-Majadahonda, Madrid, Spain.
  • Muñoz P; Azienda Ospedaliera San Camillo-Forlanini, Rome, Italy.
  • Sánchez-Carrillo C; Instituto de Investigación Sanitaria La Fe, Valencia, Spain.
  • Guinea J; Hospital Universitario y Politécnico La Fe, Valencia, Spain.
  • Escribano P; Dipartimento di Scienze Gastroenterologiche, Endocrino-Metaboliche e Nefro-Urologiche, Fondazione Policlinico Universitario A. Gemelli IRCCS, Rome, Italy.
Med Mycol ; 58(7): 887-895, 2020 Oct 01.
Article em En | MEDLINE | ID: mdl-32022851
The capacity of Candida spp. to form biofilms allows them to attach either to living or inert surfaces, promoting their persistence in hospital environments. In a previous study, we reported strain-to-strain variations in Candida spp. biofilm development, suggesting that some genotypes may be greater biofilm formers than others. In this study, we hypothesize that isolates pertaining to clusters may be found more frequently in the environment due to their ability to form biofilms compared to singleton genotypes. Two hundred and thirty-nine Candida spp. isolates (78 clusters) from candidemia patients admitted to 16 hospitals located in different cities and countries-and the same number of singleton genotypes used as controls-were tested in terms of biofilm formation using the crystal violet and the XTT reduction assays. Candida albicans clusters showed higher biofilm formation in comparison to singleton genotypes (P < .01). The biofilms formed by intra-hospital C. albicans clusters showed higher metabolic activity (P < .05). Furthermore, marked variability was found among species and type of cluster. We observed that the higher the number of isolates, the higher the variability of biofilm production by isolates within the cluster, suggesting that the production of biofilm by isolates of the same genotype is quite diverse and does not depend on the type of cluster studied. In conclusion, candidemia Candida spp. clusters-particularly in the case of C. albicans-show significantly more biomass production and metabolic activity than singleton genotypes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Candida albicans / Biofilmes / Candida tropicalis / Candida parapsilosis Tipo de estudo: Clinical_trials Limite: Humans País/Região como assunto: America do sul / Brasil / Europa Idioma: En Revista: Med Mycol Assunto da revista: MEDICINA VETERINARIA / MICROBIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Espanha País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Candida albicans / Biofilmes / Candida tropicalis / Candida parapsilosis Tipo de estudo: Clinical_trials Limite: Humans País/Região como assunto: America do sul / Brasil / Europa Idioma: En Revista: Med Mycol Assunto da revista: MEDICINA VETERINARIA / MICROBIOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Espanha País de publicação: Reino Unido