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Iron-induced derangement in hepatic Δ-5 and Δ-6 desaturation capacity and fatty acid profile leading to steatosis: Impact on extrahepatic tissues and prevention by antioxidant-rich extra virgin olive oil.
Barrera, Cynthia; Valenzuela, Rodrigo; Rincón, Miguel A; Espinosa, Alejandra; López-Arana, Sandra; González-Mañan, Daniel; Romero, Nalda; Vargas, Romina; Videla, Luis A.
Afiliação
  • Barrera C; Department of Nutrition, Faculty of Medicine, University of Chile, Independencia 1027, Casilla, Santiago 70000, Chile.
  • Valenzuela R; Department of Nutrition, Faculty of Medicine, University of Chile, Independencia 1027, Casilla, Santiago 70000, Chile. Electronic address: rvalenzuelab@med.uchile.cl.
  • Rincón MA; Institute of Nutrition and Food Technology, University of Chile, Santiago, Chile.
  • Espinosa A; Department of Medical Technology, Faculty of Medicine, University of Chile, Santiago, Chile.
  • López-Arana S; Department of Nutrition, Faculty of Medicine, University of Chile, Independencia 1027, Casilla, Santiago 70000, Chile.
  • González-Mañan D; Núcleo de Química y Bioquímica, Facultad de Ciencias, Universidad Mayor, Chile.
  • Romero N; Department of Food Science and Chemical Technology, Faculty of Chemical Sciences and Pharmacy, University of Chile, Santiago, Chile.
  • Vargas R; Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile.
  • Videla LA; Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile.
Prostaglandins Leukot Essent Fatty Acids ; 153: 102058, 2020 02.
Article em En | MEDLINE | ID: mdl-32007744
The administration of iron induces liver oxidative stress and depletion of long-chain polyunsaturated fatty acids (LCPUFAs), n-6/n-3 LCPUFA ratio enhancement and fat accumulation, which may be prevented by antioxidant-rich extra virgin olive oil (AR-EVOO) supplementation. Male Wistar rats were subjected to a control diet (50 mg iron/kg diet) or iron-rich diet (IRD; 200 mg/kg diet) with alternate AR-EVOO for 21 days. Liver fatty acid (FA) analysis was performed by gas-liquid chromatography (GLC) after lipid extraction and fractionation, besides Δ-5 desaturase (Δ-5 D) and Δ6-D mRNA expression (qPCR) and activity (GLC) measurements. The IRD significantly (p < 0.05) increased hepatic total fat, triacylglycerols, free FA contents and serum transaminases levels, with diminution in those of n-6 and n-3 LCPUFAs, higher n-6/n-3 ratios, lower unsaturation index and Δ5-D and Δ6-D activities, whereas the mRNA expression of both desaturases was enhanced over control values, changes that were prevented by concomitant AR-EVOO supplementation. N-6 and n-3 LCPUFAs were also decreased by IRD in extrahepatic tissues and normalized by AR-EVOO. In conclusion, AR-EVOO supplementation prevents IRD-induced changes in parameters related to liver FA metabolism and steatosis, an effect that may have a significant impact in the treatment of iron-related pathologies or metabolic disorders such as non-alcoholic fatty liver disease.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linoleoil-CoA Desaturase / Ácidos Graxos Dessaturases / Fígado Gorduroso / Azeite de Oliva / Ferro / Antioxidantes Limite: Animals Idioma: En Revista: Prostaglandins Leukot Essent Fatty Acids Assunto da revista: ENDOCRINOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Chile País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linoleoil-CoA Desaturase / Ácidos Graxos Dessaturases / Fígado Gorduroso / Azeite de Oliva / Ferro / Antioxidantes Limite: Animals Idioma: En Revista: Prostaglandins Leukot Essent Fatty Acids Assunto da revista: ENDOCRINOLOGIA Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Chile País de publicação: Reino Unido