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Transcriptome analysis in Parhyale hawaiensis reveal sex-specific responses to AgNP and AgCl exposure.
Artal, Mariana Coletty; Pereira, Karina Danielle; Luchessi, Augusto Ducati; Okura, Vagner Katsumi; Henry, Theodore Burdick; Marques-Souza, Henrique; de Aragão Umbuzeiro, Gisela.
Afiliação
  • Artal MC; School of Pharmaceutical Sciences, University of Sao Paulo, São Paulo, 05508-000, Brazil; School of Technology, University of Campinas, Limeira, São Paulo, 13484-332, Brazil.
  • Pereira KD; Laboratory of Biotechnology, School of Applied Sciences, University of Campinas, Limeira, São Paulo, 13484-350, Brazil; Institute of Biosciences, São Paulo State University, Rio Claro, São Paulo, 13506-900, Brazil.
  • Luchessi AD; Laboratory of Biotechnology, School of Applied Sciences, University of Campinas, Limeira, São Paulo, 13484-350, Brazil; Institute of Biosciences, São Paulo State University, Rio Claro, São Paulo, 13506-900, Brazil.
  • Okura VK; Life Sciences Core Facility (LaCTAD), University of Campinas, Campinas, São Paulo, 13083-886, Brazil.
  • Henry TB; Institute of Life and Earth Sciences, School of Energy, Geoscience, Infrastructure, and Society, Heriot-Watt University, EH14 4AS, Edinburgh, Scotland, UK; Center for Environmental Biotechnology, The University of Tennessee, 676 Dabney Hall, 1416 Circle Drive, Knoxville, TN, 37996-1605, United State
  • Marques-Souza H; Department of Biochemistry and Tissue Biology, University of Campinas, Campinas, São Paulo, 13083-970, Brazil. Electronic address: hmsouza@unicamp.br.
  • de Aragão Umbuzeiro G; School of Pharmaceutical Sciences, University of Sao Paulo, São Paulo, 05508-000, Brazil; School of Technology, University of Campinas, Limeira, São Paulo, 13484-332, Brazil. Electronic address: giselau@ft.unicamp.br.
Environ Pollut ; 260: 113963, 2020 May.
Article em En | MEDLINE | ID: mdl-32004961
Analysis of the transcriptome of organisms exposed to toxicants offers new insights for ecotoxicology, but further research is needed to enhance interpretation of results and effectively incorporate them into useful environmental risk assessments. Factors that must be clarified to improve use of transcriptomics include assessment of the effect of organism sex within the context of toxicant exposure. Amphipods are well recognized as model organisms for toxicity evaluation because of their sensitivity and amenability to laboratory conditions. To investigate whether response to metals in crustaceans differs according to sex we analyzed the amphipod Parhyale hawaiensis after exposure to AgCl and Ag nanoparticles (AgNP) via contaminated food. Gene specific analysis and whole genome transcriptional profile of male and female organisms were performed by both RT-qPCR and RNA-seq. We observed that expression of transcripts of genes glutathione transferase (GST) did not differ among AgCl and AgNP treatments. Significant differences between males and females were observed after exposure to AgCl and AgNP. Males presented twice the number of differentially expressed genes in comparison to females, and more differentially expressed were observed after exposure to AgNP than AgCl treatments in both sexes. The genes that had the greatest change in expression relative to control were those genes related to peptidase and catalytic activity and chitin and carbohydrate metabolic processes. Our study is the first to demonstrate sex specific differences in the transcriptomes of amphipods upon exposure to toxicants and emphasizes the importance of considering gender in ecotoxicology.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Prata / Anfípodes / Nanopartículas Metálicas Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Environ Pollut Assunto da revista: SAUDE AMBIENTAL Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Prata / Anfípodes / Nanopartículas Metálicas Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Environ Pollut Assunto da revista: SAUDE AMBIENTAL Ano de publicação: 2020 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido