An exploratory study of enantioselective behavior of Sol-Gel encapsulated human serum albumin using frontal analysis.

Y de la Pena, Marcela Hurtado; Herrera, Rosario Covarrubias; Vazquez, Alma Luisa Revilla

Y de la Pena, Marcela Hurtado; Herrera, Rosario Covarrubias; Vazquez, Alma Luisa Revilla.

Afiliação

Y de la Pena MH; Departamento de Quimica Analítica, Facultad de Química, UNAM, Ciudad Universitaria, Mexico D.F., Mexico/Departamento Sistemas Biologicos, UAM-Xochimilco. Calzada del Hueso, Col. Villa Quietud, Mexico D.F., Mexico.
Herrera RC; Departamento de Quimica Analítica, Facultad de Química, UNAM, Ciudad Universitaria, Mexico D.F., Mexico.
Vazquez ALR; Division de Ciencias Quimico Biologicas, Facultad de Estudios Superiores Cuautitlan, UNAM, Campo1, Av. 1° de Mayo s/n Col. Santa Maria las Torres Cuautitlán Izcalli Edo de México, Mexico.

Pak J Pharm Sci ; 32(6): 2717-2724, 2019 Nov.

Article em En | MEDLINE | ID: mdl-31969306

RESUMO

Adsorption behavior of pure enantiomers and racemic mixtures of nonsteroidal anti-inflammatory drugs (ibuprofen and naproxen) on human serum albumin (HSA) was evaluated. The HSA was immobilized by Sol-Gel technique and this biomaterial was used in a chromatographic system where frontal analysis experiments were performed at pH 7.4 and temperatures of 25°C and 37°C. The association constants for enantiomers of the drugs were determined by linear adjustment for data corrected just for dead volume. In uncorrected data for non-specific retention, an inverse ratio between the number of sites and the value of the association constant was found. The participation of non-specific retention was estimated by non-linear regression and the values of association constants (Kass), which were determined considering this information, are comparable to some values reported by other methods at 37°C: 1.4 x105 and 5.7 x104 for Ibuprofen (IBU) R and S, respectively, and 2.3 x105 and 1.8x105 for naproxen (NX) R and S, respectively.

Assuntos

Albumina Sérica Humana/farmacocinética; Anti-Inflamatórios não Esteroides/farmacocinética; Humanos; Ibuprofeno/farmacocinética; Naproxeno/farmacocinética; Transição de Fase; Albumina Sérica Humana/química; Estereoisomerismo

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Albumina Sérica Humana Limite: Humans Idioma: En Revista: Pak J Pharm Sci Assunto da revista: FARMACIA / FARMACOLOGIA / QUIMICA Ano de publicação: 2019 Tipo de documento: Article País de afiliação: México País de publicação: Paquistão

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